LOCUS CR541766 939 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0430D for
gene CA4, carbonic anhydrase IV; complete cds, incl. stopcodon.
ACCESSION CR541766
VERSION CR541766.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 939)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 939)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0430D, ORFNo 3529
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0430D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131003.01X
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_000717 (GI:9951925)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..939
/db_xref="H-InvDB:HIT000268637"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0430D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..939
/codon_start=1
/gene="CA4"
/db_xref="GOA:P22748"
/db_xref="H-InvDB:HIT000268637.12"
/db_xref="HGNC:HGNC:1375"
/db_xref="InterPro:IPR001148"
/db_xref="InterPro:IPR018338"
/db_xref="InterPro:IPR018343"
/db_xref="InterPro:IPR023561"
/db_xref="InterPro:IPR036398"
/db_xref="InterPro:IPR041874"
/db_xref="PDB:1ZNC"
/db_xref="PDB:3F7B"
/db_xref="PDB:3F7U"
/db_xref="PDB:3FW3"
/db_xref="PDB:5IPZ"
/db_xref="PDB:5JN8"
/db_xref="PDB:5JN9"
/db_xref="PDB:5JNA"
/db_xref="PDB:5JNC"
/db_xref="PDB:5KU6"
/db_xref="UniProtKB/Swiss-Prot:P22748"
/protein_id="CAG46565.1"
/translation="MRMLLALLALSAARPSASAESHWCYEVQAESSNYPCLVPVKWGG
NCQKDRQSPINIVTTKAKVDKKLGRFFFSGYDKKQTWTVQNNGHSVMMLLENKASISG
GGLPAPYQAKQLHLHWSDLPYKGSEHSLDGEHFAMEMHIVHEKEKGTSRNVKEAQDPE
DEIAVLAFLVEAGTQVNEGFQPLVEALSNIPKPEMSTTMAESSLLDLLPKEEKLRHYF
RYLGSLTTPTCDEKVVWTVFREPIQLHREQILAFSQKLYYDKEQTVSMKDNVRPLQQL
GQRTVIKSGAPGRPLPWALPALLGPMLACLLAGFLR"
BASE COUNT 220 a 271 c 279 g 169 t
ORIGIN
1 atgcggatgc tgctggcgct cctggccctc tccgcggcgc ggccatcggc cagtgcagag
61 tcacactggt gctacgaggt tcaagccgag tcctccaact acccctgctt ggtgccagtc
121 aagtggggtg gaaactgcca gaaggaccgc cagtccccca tcaacatcgt caccaccaag
181 gcaaaggtgg acaaaaaact gggacgcttc ttcttctctg gctacgataa gaagcaaacg
241 tggactgtcc aaaataacgg gcactcagtg atgatgttgc tggagaacaa ggccagcatt
301 tctggaggag gactgcctgc cccataccag gccaaacagt tgcacctgca ctggtccgac
361 ttgccatata agggctcgga gcacagcctc gatggggagc actttgccat ggagatgcac
421 atagtacatg agaaagagaa ggggacatcg aggaatgtga aagaggccca ggaccctgaa
481 gacgaaattg cggtgctggc ctttctggtg gaggctggaa cccaggtgaa cgagggcttc
541 cagccactgg tggaggcact gtctaatatc cccaaacctg agatgagcac tacgatggca
601 gagagcagcc tgttggacct gctccccaag gaggagaaac tgaggcacta cttccgctac
661 ctgggctcac tcaccacacc gacctgcgat gagaaggtcg tctggactgt gttccgggag
721 cccattcagc ttcacagaga acagatcctg gcattctctc agaagctgta ctacgacaag
781 gaacagacag tgagcatgaa ggacaatgtc aggcccctgc agcagctggg gcagcgcacg
841 gtgataaagt ccggggcccc gggtcggccg ctgccctggg ccctgcctgc cctgctgggc
901 cccatgctgg cctgcctgct ggccggcttc ctgcgatga
//