LOCUS       CR541751                 384 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834H0929D for
            gene MRPL28, mitochondrial ribosomal protein L28; complete cds,
            without stopcodon.
ACCESSION   CR541751
VERSION     CR541751.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 384)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834H0929D, ORFNo 3502
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0929D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence U19796
            we found AA exchange(s) at position (first base of changed
            triplet):
            94(glu->asp) 304(tyr->his)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..384
                     /db_xref="H-InvDB:HIT000268623"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834H0929D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>384
                     /codon_start=1
                     /gene="MRPL28"
                     /db_xref="GOA:Q6FHK1"
                     /db_xref="H-InvDB:HIT000268623.11"
                     /db_xref="InterPro:IPR026569"
                     /db_xref="UniProtKB/TrEMBL:Q6FHK1"
                     /protein_id="CAG46551.1"
                     /translation="MRTLDLIDEAYGLDFYILKTPKEDLCSKFGMDLKRGMLLRLARQ
                     DPQLHPEDPERRAAIYDKYKEFAIPEEEAEWVGLTLEEAIEKQRLLEEKDPVPLFKIH
                     VAELIQQLQQQALSEPAVVQKTASGQ"
BASE COUNT           87 a          107 c          129 g           61 t
ORIGIN      
        1 atgcggaccc tggacctcat cgatgaggct tacgggctcg acttttacat cctcaagacc
       61 ccgaaggagg acctgtgctc caagtttggg atggacctga agcgagggat gctgctgcgg
      121 cttgcccggc aggaccccca gctgcacccc gaggaccccg agcggcgggc agccatctac
      181 gacaagtaca aggaatttgc catcccagag gaggaggcag agtgggtggg cctcacgctg
      241 gaggaggcca ttgagaagca gagacttttg gaggagaagg accctgtacc cctgttcaag
      301 atccatgtgg cggagctgat ccagcagctg cagcagcagg cactgtcaga gccggcggtg
      361 gtgcagaaga cagccagtgg ccag
//