LOCUS       CR541744                 765 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834F0629D for
            gene PGAM1, phosphoglycerate mutase 1 (brain); complete cds, incl.
            stopcodon.
ACCESSION   CR541744
VERSION     CR541744.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 765)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834F0629D, ORFNo 3487
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0629D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence NM_002629 (GI:31543395)
            we found AA exchange(s) at position (first base of changed
            triplet):
            94(pro->gln)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..765
                     /db_xref="H-InvDB:HIT000268616"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834F0629D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..765
                     /codon_start=1
                     /gene="PGAM1"
                     /db_xref="GOA:Q6FHK8"
                     /db_xref="H-InvDB:HIT000268616.12"
                     /db_xref="InterPro:IPR001345"
                     /db_xref="InterPro:IPR005952"
                     /db_xref="InterPro:IPR013078"
                     /db_xref="InterPro:IPR029033"
                     /db_xref="UniProtKB/TrEMBL:Q6FHK8"
                     /protein_id="CAG46544.1"
                     /translation="MAAYKLVLIRHGESAWNLENRFSGWYDADLSQAGHEEAKRGGQA
                     LRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAE
                     TAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTI
                     ARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVY
                     ELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK"
BASE COUNT          190 a          197 c          232 g          146 t
ORIGIN      
        1 atggccgcct acaaactggt gctgatccgg cacggcgaga gcgcatggaa cctggagaac
       61 cgcttcagcg gctggtacga cgccgacctg agccaggcgg gccacgagga ggcgaagcgc
      121 ggcgggcagg cgctacgaga tgctggctat gagtttgaca tctgcttcac ctcagtgcag
      181 aagagagcga tccggaccct ctggacagtg ctagatgcca ttgatcagat gtggctgcca
      241 gtggtgagga cttggcgcct caatgagcgg cactatgggg gtctaaccgg tctcaataaa
      301 gcagaaactg ctgcaaagca tggtgaggcc caggtgaaga tctggaggcg ctcctatgat
      361 gtcccaccac ctccgatgga gcccgaccat cctttctaca gcaacatcag taaggatcgc
      421 aggtatgcag acctcacaga agatcagcta ccctcctgtg agagtctgaa ggatactatt
      481 gccagagctc tgcccttctg gaatgaagaa atagttcccc agatcaagga ggggaaacgt
      541 gtactgattg cagcccatgg caacagcctc cggggcattg tcaagcatct ggagggtctc
      601 tctgaagagg ctatcatgga gctgaacctg ccgactggta ttcccattgt ctatgaattg
      661 gacaagaact tgaagcctat caagcccatg cagtttctgg gggatgaaga gacggtgcgc
      721 aaagccatgg aagctgtggc tgcccagggc aaggccaaga agtga
//