LOCUS CR541744 765 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0629D for gene PGAM1, phosphoglycerate mutase 1 (brain); complete cds, incl. stopcodon. ACCESSION CR541744 VERSION CR541744.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 765) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 765) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834F0629D, ORFNo 3487 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0629D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_002629 (GI:31543395) we found AA exchange(s) at position (first base of changed triplet): 94(pro->gln) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..765 /db_xref="H-InvDB:HIT000268616" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834F0629D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..765 /codon_start=1 /gene="PGAM1" /db_xref="GOA:Q6FHK8" /db_xref="H-InvDB:HIT000268616.12" /db_xref="InterPro:IPR001345" /db_xref="InterPro:IPR005952" /db_xref="InterPro:IPR013078" /db_xref="InterPro:IPR029033" /db_xref="UniProtKB/TrEMBL:Q6FHK8" /protein_id="CAG46544.1" /translation="MAAYKLVLIRHGESAWNLENRFSGWYDADLSQAGHEEAKRGGQA LRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAE TAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTI ARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVY ELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK" BASE COUNT 190 a 197 c 232 g 146 t ORIGIN 1 atggccgcct acaaactggt gctgatccgg cacggcgaga gcgcatggaa cctggagaac 61 cgcttcagcg gctggtacga cgccgacctg agccaggcgg gccacgagga ggcgaagcgc 121 ggcgggcagg cgctacgaga tgctggctat gagtttgaca tctgcttcac ctcagtgcag 181 aagagagcga tccggaccct ctggacagtg ctagatgcca ttgatcagat gtggctgcca 241 gtggtgagga cttggcgcct caatgagcgg cactatgggg gtctaaccgg tctcaataaa 301 gcagaaactg ctgcaaagca tggtgaggcc caggtgaaga tctggaggcg ctcctatgat 361 gtcccaccac ctccgatgga gcccgaccat cctttctaca gcaacatcag taaggatcgc 421 aggtatgcag acctcacaga agatcagcta ccctcctgtg agagtctgaa ggatactatt 481 gccagagctc tgcccttctg gaatgaagaa atagttcccc agatcaagga ggggaaacgt 541 gtactgattg cagcccatgg caacagcctc cggggcattg tcaagcatct ggagggtctc 601 tctgaagagg ctatcatgga gctgaacctg ccgactggta ttcccattgt ctatgaattg 661 gacaagaact tgaagcctat caagcccatg cagtttctgg gggatgaaga gacggtgcgc 721 aaagccatgg aagctgtggc tgcccagggc aaggccaaga agtga //