LOCUS CR541698 1395 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0528D for
gene PFKM, phosphofructokinase, muscle; complete cds, without
stopcodon.
ACCESSION CR541698
VERSION CR541698.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1395)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1395)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0528D, ORFNo 3379
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0528D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence U24183
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1395
/db_xref="H-InvDB:HIT000268571"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0528D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1395
/codon_start=1
/gene="PFKM"
/db_xref="GOA:Q7KYX9"
/db_xref="H-InvDB:HIT000268571.13"
/db_xref="InterPro:IPR000023"
/db_xref="InterPro:IPR015912"
/db_xref="InterPro:IPR022953"
/db_xref="InterPro:IPR035966"
/db_xref="UniProtKB/TrEMBL:Q7KYX9"
/protein_id="CAG46499.1"
/translation="MGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLMECVQVTKDVT
KAMDEKKFDEALKLRGRSFMNNWEVYKLLAHVRPPVSKSGSHTVAVMNVGAPAAGMNA
AVRSTVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPK
KSFEQISANITKFNIQGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNV
PGSDFSVGADTALNTICTTCDRIKQSAAGTKRRVFIIETMGGYCGYLATMAGLAAGAD
AAYIFEEPFTIRDLQANVEHLVQKMKTTVKRGLVLRNEKCNENYTTDFIFNLYSEEGK
GIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRIFANTPDS
GCVLGMRKRALVFQPVAELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHA
HLEHITRKRSGEAAV"
BASE COUNT 345 a 325 c 413 g 312 t
ORIGIN
1 atgggtgtgg aagcagtgat ggcacttttg gaggggaccc cagatacccc agcctgtgta
61 gtgagcctct ctggtaacca ggctgtgcgc ctgcccctca tggaatgtgt ccaggtgacc
121 aaagatgtga ccaaggccat ggatgagaag aaatttgacg aagccctgaa gctgagaggc
181 cggagcttca tgaacaactg ggaggtgtac aagcttctag ctcatgtcag acccccggta
241 tctaagagtg gttcgcacac agtggctgtg atgaacgtgg gggctccggc tgcaggcatg
301 aatgctgctg ttcgctccac tgtgaggatt ggccttatcc agggcaaccg agtgctcgtt
361 gtccatgatg gtttcgaggg cctggccaag gggcagatag aggaagctgg ctggagctat
421 gttgggggct ggactggcca aggtggctct aaacttggga ctaaaaggac tctacccaag
481 aagagctttg aacagatcag tgccaatata actaagttta acattcaggg ccttgtcatc
541 attgggggct ttgaggctta cacagggggc ctggaactga tggagggcag gaagcagttt
601 gatgagctct gcatcccatt tgtggtcatt cctgctacag tctccaacaa tgtccctggc
661 tcagacttca gcgttggggc tgacacagca ctcaatacta tctgcacaac ctgtgaccgc
721 atcaagcagt cagcagctgg caccaagcgt cgggtgttta tcattgagac tatgggtggc
781 tactgtggct acctggctac catggctgga ctggcagctg gggccgatgc tgcctacatt
841 tttgaggagc ccttcaccat tcgagacctg caggcaaatg ttgaacatct ggtgcaaaag
901 atgaaaacaa ctgtgaaaag gggcttggtg ttaaggaatg aaaagtgcaa tgagaactat
961 accactgact tcattttcaa cctgtactct gaggagggga agggcatctt cgacagcagg
1021 aagaatgtgc ttggtcacat gcagcagggt gggagcccaa ccccatttga taggaatttt
1081 gccactaaga tgggcgccaa ggctatgaac tggatgtctg ggaaaatcaa agagagttac
1141 cgtaatgggc ggatctttgc caatactcca gattcgggct gtgttctggg gatgcgtaag
1201 agggctctgg tcttccaacc agtggctgag ctgaaggacc agacagattt tgagcatcga
1261 atccccaagg aacagtggtg gctgaaactg aggcccatcc tcaaaatcct agccaagtac
1321 gagattgact tggacacttc agaccatgcc cacctggagc acatcacccg gaagcggtcc
1381 ggggaagctg ccgtc
//