LOCUS CR541687 1095 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C0828D for
gene MAPK13, mitogen-activated protein kinase 13; complete cds,
without stopcodon.
ACCESSION CR541687
VERSION CR541687.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1095)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1095)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C0828D, ORFNo 3343
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0828D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131045.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_002754 (GI:20986527)
we found AA exchange(s) at position (first base of changed
triplet):
874(asp->gly) 1030(lys->asn)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1095
/db_xref="H-InvDB:HIT000268560"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C0828D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1095
/codon_start=1
/gene="MAPK13"
/db_xref="GOA:Q6FHR4"
/db_xref="H-InvDB:HIT000268560.13"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR003527"
/db_xref="InterPro:IPR008352"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR017441"
/db_xref="InterPro:IPR038785"
/db_xref="UniProtKB/TrEMBL:Q6FHR4"
/protein_id="CAG46488.1"
/translation="MSLIRKKGFYKQDVNKTAWELPKTYVSPTHVGSGAYGSVCSAID
KRSGEKVAIKKLSRPFQSEIFAKRAYRELLLLKHMQHENVIGLLDVFTPASSLRNFYD
FYLVMPFMQTDLQKIMGMEFSEEKIQYLVYQMLKGLKYIHSAGVVHRDLKPGNLAVNE
DCELKILDFGLARHADAEMTGYVVTRWYRAPEVILSWMHYNQTVDIWSVGCIMAEMLT
GKTLFKGKDYLDQLTQILKVTGVPGTEFVQKLNDKAAKSYIQSLPQTPRKDFTQLFPR
ASPQAADLLEKMLELGVDKRLTAAQALTHPFFEPFRDPEEETEAQQPFDDSLEHEKLT
VDEWKQHIYNEIVNFSPIARKDSRRRSGMKL"
BASE COUNT 257 a 310 c 324 g 204 t
ORIGIN
1 atgagcctca tccggaaaaa gggcttctac aagcaggacg tcaacaagac cgcctgggag
61 ctgcccaaga cctacgtgtc cccgacgcac gtcggcagcg gggcctatgg ctccgtgtgc
121 tcggccatcg acaagcggtc gggggagaag gtggccatca agaagctgag ccgacccttt
181 cagtccgaga tcttcgccaa gcgcgcctac cgggagctgc tgctgctgaa gcacatgcag
241 catgagaacg tcattgggct cctggatgtc ttcaccccag cctcctccct gcgcaacttc
301 tatgacttct acctggtgat gcccttcatg cagacggatc tgcagaagat catggggatg
361 gagttcagtg aggagaagat ccagtacctg gtgtatcaga tgctcaaagg ccttaagtac
421 atccactctg ctggggtcgt gcacagggac ctgaagccag gcaacctggc tgtgaatgag
481 gactgtgaac tgaagattct ggattttggg ctggcgcgac atgcagacgc cgagatgact
541 ggctacgtgg tgacccgctg gtaccgagcc cccgaggtga tcctcagctg gatgcactac
601 aaccagacag tggacatctg gtctgtgggc tgtatcatgg cagagatgct gacagggaaa
661 actctgttca aggggaaaga ttacctggac cagctgaccc agatcctgaa agtgaccggg
721 gtgcctggca cggagtttgt gcagaagctg aacgacaaag cggccaaatc ctacatccag
781 tccctgccac agacccccag gaaggatttc actcagctgt tcccacgggc cagcccccag
841 gctgcggacc tgctggagaa gatgctggag ctaggcgtgg acaagcgcct gacggccgcg
901 caggccctca cccatccctt ctttgaaccc ttccgggacc ctgaggaaga gacggaggcc
961 cagcagccgt ttgatgattc cttagaacac gagaaactca cagtggatga atggaagcag
1021 cacatctaca atgagattgt gaacttcagc cccattgccc ggaaggactc acggcgccgg
1081 agtggcatga agctg
//