LOCUS CR541685 606 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0728D for
gene PTTG1, pituitary tumor-transforming 1; complete cds, without
stopcodon.
ACCESSION CR541685
VERSION CR541685.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 606)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 606)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0728D, ORFNo 3338
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0728D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131040.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_004219 (GI:11038651)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..606
/db_xref="H-InvDB:HIT000268558"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0728D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>606
/codon_start=1
/gene="PTTG1"
/db_xref="GOA:Q6IAL9"
/db_xref="H-InvDB:HIT000268558.12"
/db_xref="InterPro:IPR006940"
/db_xref="UniProtKB/TrEMBL:Q6IAL9"
/protein_id="CAG46486.1"
/translation="MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTP
RFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKS
SVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQ
LGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI"
BASE COUNT 170 a 151 c 135 g 150 t
ORIGIN
1 atggctactc tgatctatgt tgataaggaa aacggagaac caggcacccg tgtggttgct
61 aaggatgggc tgaagctggg gtctggacct tcaatcaaag ccttagatgg gagatctcaa
121 gtttcaacac cacgttttgg caaaacgttc gatgccccac cagccttacc taaagctact
181 agaaaggctt tgggaactgt caacagagct acagaaaagt ctgtaaagac caagggaccc
241 ctcaaacaaa aacagccaag cttttctgcc aaaaagatga ctgagaagac tgttaaagca
301 aaaagctctg ttcctgcctc agatgatgcc tatccagaaa tagaaaaatt ctttcccttc
361 aatcctctag actttgagag ttttgacctg cctgaagagc accagattgc gcacctcccc
421 ttgagtggag tgcctctcat gatccttgac gaggagagag agcttgaaaa gctgtttcag
481 ctgggccccc cttcacctgt gaagatgccc tctccaccat gggaatccaa tctgttacag
541 tctccttcaa gcattctgtc gaccctggat gttgaattgc cacctgtttg ctgtgacata
601 gatatt
//