LOCUS CR541663 606 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0227D for
gene RNP24, coated vesicle membrane protein; complete cds, incl.
stopcodon.
ACCESSION CR541663
VERSION CR541663.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 606)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 606)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0227D, ORFNo 3266
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0227D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131065.01X
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_006815 (GI:21314646)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..606
/db_xref="H-InvDB:HIT000268536"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0227D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..606
/codon_start=1
/gene="RNP24"
/db_xref="GOA:Q6FHT8"
/db_xref="H-InvDB:HIT000268536.13"
/db_xref="InterPro:IPR009038"
/db_xref="InterPro:IPR015720"
/db_xref="InterPro:IPR036598"
/db_xref="UniProtKB/TrEMBL:Q6FHT8"
/protein_id="CAG46464.1"
/translation="MVTLAELLVLLAALLATVSGYFVSIDAHAEECFFERVTSGTKMG
LIFEVAEGGFLDIDVEITGPDNKGIYKGDRESSGKYTFAAHMDGTYKFCFSNRMSTMT
PKIVMFTIDIGEAPKGQDMETEAHQNKLEEMINELAVAMTAVKHEQEYMEVRERIHRA
INDNTNSRVVLWSFFEALVLVAMTLGQIYYLKRFFEVRRVV"
BASE COUNT 176 a 131 c 160 g 139 t
ORIGIN
1 atggtgacgc ttgctgaact gctggtgctc ctggccgctc tcctggccac ggtctcgggc
61 tatttcgtta gcatcgacgc ccatgctgaa gagtgcttct ttgagcgggt cacctcgggc
121 accaagatgg gcctcatctt cgaggtggcg gagggcggct tcctggacat cgacgtggag
181 attacaggac cagataacaa aggaatttac aaaggagaca gagaatccag tgggaaatac
241 acatttgctg ctcacatgga tggaacatac aaattttgtt ttagtaaccg gatgtccacc
301 atgactccaa aaatagtgat gttcaccatt gatattgggg aggctccaaa aggacaagat
361 atggaaacag aagctcacca gaacaagcta gaagaaatga tcaatgagct agcagtggcg
421 atgacagctg taaagcacga acaggaatac atggaagtcc gggagagaat acacagagcc
481 atcaacgaca acacaaacag cagagtggtc ctttggtcct tctttgaagc tcttgttcta
541 gttgccatga cattgggaca gatctactac ctgaagagat tttttgaagt ccggagagtt
601 gtttaa
//