LOCUS CR541660 1146 bp mRNA linear HUM 29-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1127D for
gene GJA1, gap junction protein, alpha 1, 43kDa (connexin 43);
complete cds, without stopcodon.
ACCESSION CR541660
VERSION CR541660.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1146)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1146)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1127D, ORFNo 3250
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1127D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
Contact RZPD (customer.service@rzpd.de) for further information.
Clone name at Harvard Institute of Proteomics
(www.hip.harvard.edu):
FLH131079.01L
This CDS clone is part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics (HIP) and RZPD.
This CDS has been cloned without stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
The clone is validated by full sequence check.
Compared to the reference sequence NM_000165 (GI:4755136)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1146
/db_xref="H-InvDB:HIT000268533"
/organism="Homo sapiens"
/lab_host="DH5Alpha"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1127D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..>1146
/codon_start=1
/gene="GJA1"
/db_xref="GOA:P17302"
/db_xref="H-InvDB:HIT000268533.12"
/db_xref="HGNC:HGNC:4274"
/db_xref="InterPro:IPR000500"
/db_xref="InterPro:IPR002261"
/db_xref="InterPro:IPR013092"
/db_xref="InterPro:IPR013124"
/db_xref="InterPro:IPR017990"
/db_xref="InterPro:IPR019570"
/db_xref="InterPro:IPR034634"
/db_xref="InterPro:IPR035091"
/db_xref="InterPro:IPR038359"
/db_xref="PDB:2LL2"
/db_xref="UniProtKB/Swiss-Prot:P17302"
/protein_id="CAG46461.1"
/translation="MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESA
WGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRK
EEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISIL
FKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSL
VSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTA
PLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQP
FDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI"
BASE COUNT 290 a 279 c 282 g 295 t
ORIGIN
1 atgggtgact ggagcgcctt aggcaaactc cttgacaagg ttcaagccta ctcaactgct
61 ggagggaagg tgtggctgtc agtacttttc attttccgaa tcctgctgct ggggacagcg
121 gttgagtcag cctggggaga tgagcagtct gcctttcgtt gtaacactca gcaacctggt
181 tgtgaaaatg tctgctatga caagtctttc ccaatctctc atgtgcgctt ctgggtcctg
241 cagatcatat ttgtgtctgt acccacactc ttgtacctgg ctcatgtgtt ctatgtgatg
301 cgaaaggaag agaaactgaa caagaaagag gaagaactca aggttgccca aactgatggt
361 gtcaatgtgg acatgcactt gaagcagatt gagataaaga agttcaagta cggtattgaa
421 gagcatggta aggtgaaaat gcgagggggg ttgctgcgaa cctacatcat cagtatcctc
481 ttcaagtcta tctttgaggt ggccttcttg ctgatccagt ggtacatcta tggattcagc
541 ttgagtgctg tttacacttg caaaagagat ccctgcccac atcaggtgga ctgtttcctc
601 tctcgcccca cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtcc
661 ctggccttga atatcattga actcttctat gttttcttca agggcgttaa ggatcgggtt
721 aagggaaaga gcgaccctta ccatgcgacc agtggtgcgc tgagccctgc caaagactgt
781 gggtctcaaa aatatgctta tttcaatggc tgctcctcac caaccgctcc cctctcgcct
841 atgtctcctc ctgggtacaa gctggttact ggcgacagaa acaattcttc ttgccgcaat
901 tacaacaagc aagcaagtga gcaaaactgg gctaattaca gtgcagaaca aaatcgaatg
961 gggcaggcgg gaagcaccat ctctaactcc catgcacagc cttttgattt ccccgatgat
1021 aaccagaatt ctaaaaaact agctgctgga catgaattac agccactagc cattgtggac
1081 cagcgacctt caagcagagc cagcagtcgt gccagcagca gacctcggcc tgatgacctg
1141 gagatc
//