LOCUS CR541659 762 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1027D for gene PGAM1, phosphoglycerate mutase 1 (brain); complete cds, without stopcodon. ACCESSION CR541659 VERSION CR541659.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 762) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 762) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D1027D, ORFNo 3249 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1027D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence NM_002629 (GI:31543395) we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..762 /db_xref="H-InvDB:HIT000268532" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D1027D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>762 /codon_start=1 /gene="PGAM1" /db_xref="GOA:Q6FHU2" /db_xref="H-InvDB:HIT000268532.12" /db_xref="InterPro:IPR001345" /db_xref="InterPro:IPR005952" /db_xref="InterPro:IPR013078" /db_xref="InterPro:IPR029033" /db_xref="UniProtKB/TrEMBL:Q6FHU2" /protein_id="CAG46460.1" /translation="MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQA LRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAE TAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTI ARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVY ELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK" BASE COUNT 188 a 198 c 231 g 145 t ORIGIN 1 atggccgcct acaaactggt gctgatccgg cacggcgaga gcgcatggaa cctggagaac 61 cgcttcagcg gctggtacga cgccgacctg agcccggcgg gccacgagga ggcgaagcgc 121 ggcgggcagg cgctacgaga tgctggctat gagtttgaca tctgcttcac ctcagtgcag 181 aagagagcga tccggaccct ctggacagtg ctagatgcca ttgatcagat gtggctgcca 241 gtggtgagga cttggcgcct caatgagcgg cactatgggg gtctaaccgg tctcaataaa 301 gcagaaactg ctgcaaagca tggtgaggcc caggtgaaga tctggaggcg ctcctatgat 361 gtcccaccac ctccgatgga gcccgaccat cctttctaca gcaacatcag taaggatcgc 421 aggtatgcag acctcacaga agatcagcta ccctcctgtg agagtctgaa ggatactatt 481 gccagagctc tgcccttctg gaatgaagaa atagttcccc agatcaagga ggggaaacgt 541 gtactgattg cagcccatgg caacagcctc cggggcattg tcaagcatct ggagggtctc 601 tctgaagagg ctatcatgga gctgaacctg ccgactggta ttcccattgt ctatgaattg 661 gacaagaact tgaagcctat caagcccatg cagtttctgg gggatgaaga gacggtgcgc 721 aaagccatgg aagctgtggc tgcccagggc aaggccaaga ag //