LOCUS CR541648 393 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0527D for gene MIA, melanoma inhibitory activity; complete cds, without stopcodon. ACCESSION CR541648 VERSION CR541648.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 393) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 393) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834A0527D, ORFNo 3226 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0527D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; Contact RZPD (customer.service@rzpd.de) for further information. Clone name at Harvard Institute of Proteomics (www.hip.harvard.edu): FLH131070.01L This CDS clone is part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics (HIP) and RZPD. This CDS has been cloned without stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att The clone is validated by full sequence check. Compared to the reference sequence BT007044 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..393 /db_xref="H-InvDB:HIT000268521" /organism="Homo sapiens" /lab_host="DH5Alpha" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834A0527D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..>393 /codon_start=1 /gene="MIA" /db_xref="GOA:Q16674" /db_xref="H-InvDB:HIT000268521.12" /db_xref="HGNC:HGNC:7076" /db_xref="InterPro:IPR001452" /db_xref="InterPro:IPR036028" /db_xref="PDB:1HJD" /db_xref="PDB:1I1J" /db_xref="PDB:1K0X" /db_xref="PDB:5IXB" /db_xref="UniProtKB/Swiss-Prot:Q16674" /protein_id="CAG46449.1" /translation="MARSLVCLGVIILLSAFSGPGVRGGPMPKLADRKLCADQECSHP ISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFWGGSVQGDYYGDLAARLGY FPSSIVREDQTLKPGKVDVKTDKWDFYCQ" BASE COUNT 70 a 111 c 120 g 92 t ORIGIN 1 atggcccggt ccctggtgtg ccttggtgtc atcatcttgc tgtctgcctt ctccggacct 61 ggtgtcaggg gtggtcctat gcccaagctg gctgaccgga agctgtgtgc ggaccaggag 121 tgcagccacc ctatctccat ggctgtggcc cttcaggact acatggcccc cgactgccga 181 ttcctgacca ttcaccgggg ccaagtggtg tatgtcttct ccaagctgaa gggccgtggg 241 cggctcttct ggggaggcag cgttcaggga gattactatg gagatctggc tgctcgcctg 301 ggctatttcc ccagtagcat tgtccgagag gaccagaccc tgaaacctgg caaagtcgat 361 gtgaagacag acaaatggga tttctactgc cag //