LOCUS       CR541648                 393 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834A0527D for
            gene MIA, melanoma inhibitory activity; complete cds, without
            stopcodon.
ACCESSION   CR541648
VERSION     CR541648.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 393)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (28-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834A0527D, ORFNo 3226
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0527D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            Contact RZPD (customer.service@rzpd.de) for further information.
            Clone name at Harvard Institute of Proteomics
            (www.hip.harvard.edu):
            FLH131070.01L
            
            This CDS clone is part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics (HIP) and RZPD.
            This CDS has been cloned without stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The last codon is followed by the 3' att site: GACCCAGCTTTCTT..att
            The clone is validated by full sequence check.
            Compared to the reference sequence BT007044
            we did not find any amino acid exchanges.
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..393
                     /db_xref="H-InvDB:HIT000268521"
                     /organism="Homo sapiens"
                     /lab_host="DH5Alpha"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834A0527D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..>393
                     /codon_start=1
                     /gene="MIA"
                     /db_xref="GOA:Q16674"
                     /db_xref="H-InvDB:HIT000268521.12"
                     /db_xref="HGNC:HGNC:7076"
                     /db_xref="InterPro:IPR001452"
                     /db_xref="InterPro:IPR036028"
                     /db_xref="PDB:1HJD"
                     /db_xref="PDB:1I1J"
                     /db_xref="PDB:1K0X"
                     /db_xref="PDB:5IXB"
                     /db_xref="UniProtKB/Swiss-Prot:Q16674"
                     /protein_id="CAG46449.1"
                     /translation="MARSLVCLGVIILLSAFSGPGVRGGPMPKLADRKLCADQECSHP
                     ISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFWGGSVQGDYYGDLAARLGY
                     FPSSIVREDQTLKPGKVDVKTDKWDFYCQ"
BASE COUNT           70 a          111 c          120 g           92 t
ORIGIN      
        1 atggcccggt ccctggtgtg ccttggtgtc atcatcttgc tgtctgcctt ctccggacct
       61 ggtgtcaggg gtggtcctat gcccaagctg gctgaccgga agctgtgtgc ggaccaggag
      121 tgcagccacc ctatctccat ggctgtggcc cttcaggact acatggcccc cgactgccga
      181 ttcctgacca ttcaccgggg ccaagtggtg tatgtcttct ccaagctgaa gggccgtggg
      241 cggctcttct ggggaggcag cgttcaggga gattactatg gagatctggc tgctcgcctg
      301 ggctatttcc ccagtagcat tgtccgagag gaccagaccc tgaaacctgg caaagtcgat
      361 gtgaagacag acaaatggga tttctactgc cag
//