LOCUS CR536579 570 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0522D for gene HRAS, v-Ha-ras Harvey rat sarcoma viral oncogene homolog; complete cds, incl. stopcodon. ACCESSION CR536579 VERSION CR536579.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 570) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 570) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834G0522D, ORFNo 3194 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0522D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: (stop)GACCCAGCTTTCTT..att Compared to the reference sequence AF493916 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..570 /db_xref="H-InvDB:HIT000268515" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834G0522D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..570 /codon_start=1 /gene="HRAS" /db_xref="GOA:P01112" /db_xref="H-InvDB:HIT000268515.12" /db_xref="HGNC:HGNC:5173" /db_xref="InterPro:IPR001806" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR020849" /db_xref="InterPro:IPR027417" /db_xref="PDB:121P" /db_xref="PDB:1AA9" /db_xref="PDB:1AGP" /db_xref="PDB:1BKD" /db_xref="PDB:1CLU" /db_xref="PDB:1CRP" /db_xref="PDB:1CRQ" /db_xref="PDB:1CRR" /db_xref="PDB:1CTQ" /db_xref="PDB:1GNP" /db_xref="PDB:1GNQ" /db_xref="PDB:1GNR" /db_xref="PDB:1HE8" /db_xref="PDB:1IAQ" /db_xref="PDB:1IOZ" /db_xref="PDB:1JAH" /db_xref="PDB:1JAI" /db_xref="PDB:1K8R" /db_xref="PDB:1LF0" /db_xref="PDB:1LF5" /db_xref="PDB:1LFD" /db_xref="PDB:1NVU" /db_xref="PDB:1NVV" /db_xref="PDB:1NVW" /db_xref="PDB:1NVX" /db_xref="PDB:1P2S" /db_xref="PDB:1P2T" /db_xref="PDB:1P2U" /db_xref="PDB:1P2V" /db_xref="PDB:1PLJ" /db_xref="PDB:1PLK" /db_xref="PDB:1PLL" /db_xref="PDB:1Q21" /db_xref="PDB:1QRA" /db_xref="PDB:1RVD" /db_xref="PDB:1WQ1" /db_xref="PDB:1XCM" /db_xref="PDB:1XD2" /db_xref="PDB:1XJ0" /db_xref="PDB:1ZVQ" /db_xref="PDB:1ZW6" /db_xref="PDB:221P" /db_xref="PDB:2C5L" /db_xref="PDB:2CE2" /db_xref="PDB:2CL0" /db_xref="PDB:2CL6" /db_xref="PDB:2CL7" /db_xref="PDB:2CLC" /db_xref="PDB:2CLD" /db_xref="PDB:2EVW" /db_xref="PDB:2GDP" /db_xref="PDB:2LCF" /db_xref="PDB:2LWI" /db_xref="PDB:2N42" /db_xref="PDB:2N46" /db_xref="PDB:2Q21" /db_xref="PDB:2QUZ" /db_xref="PDB:2RGA" /db_xref="PDB:2RGB" /db_xref="PDB:2RGC" /db_xref="PDB:2RGD" /db_xref="PDB:2RGE" /db_xref="PDB:2RGG" /db_xref="PDB:2UZI" /db_xref="PDB:2VH5" /db_xref="PDB:2X1V" /db_xref="PDB:3DDC" /db_xref="PDB:3I3S" /db_xref="PDB:3K8Y" /db_xref="PDB:3K9L" /db_xref="PDB:3K9N" /db_xref="PDB:3KKM" /db_xref="PDB:3KKN" /db_xref="PDB:3KUD" /db_xref="PDB:3L8Y" /db_xref="PDB:3L8Z" /db_xref="PDB:3LBH" /db_xref="PDB:3LBI" /db_xref="PDB:3LBN" /db_xref="PDB:3LO5" /db_xref="PDB:3OIU" /db_xref="PDB:3OIV" /db_xref="PDB:3OIW" /db_xref="PDB:3RRY" /db_xref="PDB:3RRZ" /db_xref="PDB:3RS0" /db_xref="PDB:3RS2" /db_xref="PDB:3RS3" /db_xref="PDB:3RS4" /db_xref="PDB:3RS5" /db_xref="PDB:3RS7" /db_xref="PDB:3RSL" /db_xref="PDB:3RSO" /db_xref="PDB:3TGP" /db_xref="PDB:421P" /db_xref="PDB:4DLR" /db_xref="PDB:4DLS" /db_xref="PDB:4DLT" /db_xref="PDB:4DLU" /db_xref="PDB:4DLV" /db_xref="PDB:4DLW" /db_xref="PDB:4DLX" /db_xref="PDB:4DLY" /db_xref="PDB:4DLZ" /db_xref="PDB:4DST" /db_xref="PDB:4DSU" /db_xref="PDB:4EFL" /db_xref="PDB:4EFM" /db_xref="PDB:4EFN" /db_xref="PDB:4G0N" /db_xref="PDB:4G3X" /db_xref="PDB:4K81" /db_xref="PDB:4L9S" /db_xref="PDB:4L9W" /db_xref="PDB:4NYI" /db_xref="PDB:4NYJ" /db_xref="PDB:4NYM" /db_xref="PDB:4Q21" /db_xref="PDB:4RSG" /db_xref="PDB:4URU" /db_xref="PDB:4URV" /db_xref="PDB:4URW" /db_xref="PDB:4URX" /db_xref="PDB:4URY" /db_xref="PDB:4URZ" /db_xref="PDB:4US0" /db_xref="PDB:4US1" /db_xref="PDB:4US2" /db_xref="PDB:4XVQ" /db_xref="PDB:4XVR" /db_xref="PDB:521P" /db_xref="PDB:5B2Z" /db_xref="PDB:5B30" /db_xref="PDB:5E95" /db_xref="PDB:5P21" /db_xref="PDB:5VBE" /db_xref="PDB:5VBZ" /db_xref="PDB:5WDO" /db_xref="PDB:5WDP" /db_xref="PDB:5WDQ" /db_xref="PDB:5WFO" /db_xref="PDB:5WFP" /db_xref="PDB:5WFQ" /db_xref="PDB:5WFR" /db_xref="PDB:5WPL" /db_xref="PDB:5X9S" /db_xref="PDB:5ZC6" /db_xref="PDB:621P" /db_xref="PDB:6AMB" /db_xref="PDB:6AXG" /db_xref="PDB:6BVI" /db_xref="PDB:6BVJ" /db_xref="PDB:6BVK" /db_xref="PDB:6BVL" /db_xref="PDB:6BVM" /db_xref="PDB:6CUO" /db_xref="PDB:6CUP" /db_xref="PDB:6CUR" /db_xref="PDB:6D55" /db_xref="PDB:6D56" /db_xref="PDB:6D59" /db_xref="PDB:6D5E" /db_xref="PDB:6D5G" /db_xref="PDB:6D5H" /db_xref="PDB:6D5J" /db_xref="PDB:6D5L" /db_xref="PDB:6D5M" /db_xref="PDB:6D5V" /db_xref="PDB:6D5W" /db_xref="PDB:6DZH" /db_xref="PDB:6Q21" /db_xref="PDB:721P" /db_xref="PDB:821P" /db_xref="UniProtKB/Swiss-Prot:P01112" /protein_id="CAG38816.1" /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQV VIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKR VKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLV REIRQHKLRKLNPPDESGPGCMSCKCVLS" BASE COUNT 126 a 151 c 187 g 106 t ORIGIN 1 atgacggaat ataagctggt ggtggtgggc gccggcggtg tgggcaagag tgcgctgacc 61 atccagctga tccagaacca ctttgtggac gaatacgacc ccactataga ggattcctac 121 cggaagcagg tggtcattga tggggagacg tgcctgttgg acatcctgga taccgccggc 181 caggaggagt acagcgccat gcgggaccag tacatgcgca ccggggaggg cttcctgtgt 241 gtgtttgcca tcaacaacac caagtctttt gaggacatcc accagtacag ggagcagatc 301 aaacgggtga aggactcgga tgacgtgccc atggtgctgg tggggaacaa gtgtgacctg 361 gctgcacgca ctgtggaatc tcggcaggct caggacctcg cccgaagcta cggcatcccc 421 tacatcgaga cctcggccaa gacccggcag ggagtggagg atgccttcta cacgttggtg 481 cgtgagatcc ggcagcacaa gctgcggaag ctgaaccctc ctgatgagag tggccccggc 541 tgcatgagct gcaagtgtgt gctctcctga //