LOCUS CR536572 1674 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0922D for
gene TCF2, transcription factor 2, hepatic; LF-B3; variant hepatic
nuclear factor; complete cds, incl. stopcodon.
ACCESSION CR536572
VERSION CR536572.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1674)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1674)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H0922D, ORFNo 3187
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0922D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_000458 (gi4507396)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1674
/db_xref="H-InvDB:HIT000268508"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H0922D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1674
/codon_start=1
/gene="TCF2"
/db_xref="GOA:Q6FHW6"
/db_xref="H-InvDB:HIT000268508.12"
/db_xref="InterPro:IPR001356"
/db_xref="InterPro:IPR006897"
/db_xref="InterPro:IPR006899"
/db_xref="InterPro:IPR009057"
/db_xref="InterPro:IPR010982"
/db_xref="InterPro:IPR023219"
/db_xref="InterPro:IPR039066"
/db_xref="UniProtKB/TrEMBL:Q6FHW6"
/protein_id="CAG38809.1"
/translation="MVSKLTSLQQELLSALLSSGVTKEVLVQALEELLPSPNFGVKLE
TLPLSPGSGAEPDTKPVFHTLTNGHAKGRLSGDEGSEDGDDYDTPPILKELQALNTEE
AAEQRAEVDRMLSEDPWRAAKMIKGYMQQHNIPQREVVDVTGLNQSHLSQHLNKGTPM
KTQKRAALYTWYVRKQREILRQFNQTVQSSGNMTDKSSQDQLLFLFPEFSQQSHGPGQ
SDDACSEPTNKKMRRNRFKWGPASQQILYQAYDRQKNPSKEEREALVEECNRAECLQR
GVSPSKAHGLGSNLVTEVRVYNWFANRRKEEAFRQKLAMDAYSSNQTHSLNPLLSHGS
PHHQPSSSPPNKLSGVRYSQQGNNEITSSSTISHHGNSAMVTSQSVLQQVSPASLDPG
HNLLSPDGKMISVSGGGLPPVSTLTNIHSLSHHNPQQSQNLIMTPLSGVMAIAQSLNT
SQAQSVPVINSVAGSLAALQPVQFSQQLHSPHQQPLMQQSPGSHMAQQPFMAAVTQLQ
NSHMYAHKQEPPQYSHTSRFPSAMVVTDTSSISTLTNMSSSKQCPLQAW"
BASE COUNT 414 a 559 c 419 g 282 t
ORIGIN
1 atggtgtcca agctcacgtc gctccagcaa gaactcctga gcgccctgct gagctccggg
61 gtcaccaagg aggtgctggt tcaggccttg gaggagttgc tgccatcccc gaacttcggg
121 gtgaagctgg agacgctgcc cctgtcccct ggcagcgggg ccgagcccga caccaagccg
181 gtcttccata ctctcaccaa cggccacgcc aagggccgct tgtccggcga cgagggctcc
241 gaggacggcg acgactatga cacacctccc atcctcaagg agctgcaggc gctcaacacc
301 gaggaggcgg cggagcagcg ggcggaggtg gaccggatgc tcagtgagga cccttggagg
361 gctgctaaaa tgatcaaggg ttacatgcag caacacaaca tcccccagag ggaggtggtc
421 gatgtcaccg gcctgaacca gtcgcacctc tcccagcatc tcaacaaggg cacccctatg
481 aagacccaga agcgtgccgc tctgtacacc tggtacgtca gaaagcaacg agagatcctc
541 cgacaattca accagacagt ccagagttct ggaaatatga cagacaaaag cagtcaggat
601 cagctgctgt ttctctttcc agagttcagt caacagagcc atgggcctgg gcagtccgat
661 gatgcctgct ctgagcccac caacaagaag atgcgccgca accggttcaa atgggggccc
721 gcgtcccagc aaatcttgta ccaggcctac gatcggcaaa agaaccccag caaggaagag
781 agagaggcct tagtggagga atgcaacagg gcagaatgtt tgcagcgagg ggtgtccccc
841 tccaaagccc acggcctggg ctccaacttg gtcactgagg tccgtgtcta caactggttt
901 gcaaaccgca ggaaggagga ggcattccgg caaaagctgg ccatggacgc ctatagctcc
961 aaccagactc acagcctgaa ccctctgctc tcccacggct ccccccacca ccagcccagc
1021 tcctctcctc caaacaagct gtcaggagtg cgctacagcc agcagggaaa caatgagatc
1081 acttcctcct caacaatcag tcaccatggc aacagcgcca tggtgaccag ccagtcggtt
1141 ttacagcaag tctccccagc cagcctggac ccaggccaca atctcctctc acctgatggt
1201 aaaatgatct cagtctcagg aggaggtttg cccccagtca gcaccttgac gaatatccac
1261 agcctctccc accataatcc ccagcaatct caaaacctca tcatgacacc cctctctgga
1321 gtcatggcaa ttgcacaaag cctcaacacc tcccaagcac agagtgtccc tgtcatcaac
1381 agtgtggccg gcagcctggc agccctgcag cccgtccagt tctcccagca gctgcacagc
1441 cctcaccagc agcccctcat gcagcagagc ccaggcagcc acatggccca gcagcccttc
1501 atggcagctg tgactcagct gcagaactca cacatgtacg cacacaagca ggaacccccc
1561 cagtattccc acacctcccg gtttccatct gcaatggtgg tcacagatac cagcagcatc
1621 agtacactca ccaacatgtc ttcaagtaaa cagtgtcctc tacaagcctg gtga
//