LOCUS CR536566 2238 bp mRNA linear HUM 21-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0922D for
gene RPS6KA6, ribosomal protein S6 kinase, 90kDa, polypeptide 6;
complete cds, incl. stopcodon.
ACCESSION CR536566
VERSION CR536566.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2238)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 2238)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0922D, ORFNo 3174
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0922D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_014496 (gi7657525)
we found AA exchange(s) at position (first base of changed
triplet):
346(asp->gly) 883(phe->leu) 1021(arg->gly)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..2238
/db_xref="H-InvDB:HIT000268502"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0922D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..2238
/codon_start=1
/gene="RPS6KA6"
/db_xref="GOA:Q9UK32"
/db_xref="H-InvDB:HIT000268502.12"
/db_xref="HGNC:HGNC:10435"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR000961"
/db_xref="InterPro:IPR008271"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR016239"
/db_xref="InterPro:IPR017441"
/db_xref="InterPro:IPR017892"
/db_xref="InterPro:IPR041906"
/db_xref="PDB:6G76"
/db_xref="PDB:6G77"
/db_xref="PDB:6G78"
/db_xref="UniProtKB/Swiss-Prot:Q9UK32"
/protein_id="CAG38803.1"
/translation="MLPFAPQDEPWDREMEVFSGGGASSGEVNGLKMVDEPMEEGEAD
SCHDEGVVKEIPITHHVKEGYEKADPAQFELLKVLGQGSFGKVFLVRKKTGPDAGQLY
AMKVLKKASLKVRGRVRTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDV
FTRLSKEVLFTEEDVKFYLAELALALDHLHQLGIVYRDLKPENILLDEIGHIKLTDFG
LSKESVDQEKKAYSFCGTVEYMAPEVVNRRGHSQSADWWSYGVLMFEMLTGTLPFQGK
DRNETMNMILKAKLGMPQLLSAEAQSLLRMLFKRNPANRLGSEGVEEIKRHLFFANID
WDKLYKGEVQPPFKPASGKPDDTFCFDPEFTAKTPKDSPGLPASANAHQLFKGFSFVA
TSIAEEYKITPITSANVLPIVQINGNAAQFGEVYELKEDIGVGSYSVCKRCIHATTNM
EFAVKIIDKSKRDPSEEIEILMRYGQHPNIITLKDVFDDGRYVYLVTDLMKGGELLDR
ILKQKCFSEREASDILYVISKTVDYLHCQGVVHRDLKPSNILYMDESASADSIRICDF
GFAKQLRGENGLLLTPCYTANFVAPEVLMQQGYDAACDIWSLGVLFYTMLAGYTPFAN
GPNDTPEEILLRIGNGKFSLSGGNWDNISDGAKDLLSHMLHMDPHQRYTAEQILKHSW
ITHRDQLPNDQPKRNDVSHVVKGAMVATYSALTHKTFQPVLEPVAASSLAQRRSMKKR
TSTGL"
BASE COUNT 699 a 399 c 513 g 627 t
ORIGIN
1 atgctaccat tcgctcctca ggacgagccc tgggaccgag aaatggaagt gttcagcggc
61 ggcggcgcga gcagcggcga ggtaaatggt cttaaaatgg ttgatgagcc aatggaagag
121 ggagaagcag attcttgtca tgatgaagga gttgttaaag aaatccctat tactcatcat
181 gttaaggaag gctatgagaa agcagatcct gcacagtttg agttgctcaa ggttcttggt
241 caggggtcat ttggaaaggt ttttcttgtt agaaagaaga ccggtcctga tgctgggcag
301 ctctatgcaa tgaaggtgtt aaaaaaagcc tctttaaaag ttcgaggcag agttcggaca
361 aagatggaga gggatatact ggtggaagta aatcatccat ttattgtcaa attgcactat
421 gcctttcaga ctgaagggaa actgtactta atactggatt ttctcagggg aggagatgtt
481 ttcacaagat tatccaaaga ggttctgttt acagaggaag atgtgaaatt ctacctcgca
541 gaactggccc ttgctttgga tcatctgcac caattaggaa ttgtttatag agacctgaag
601 ccagaaaaca ttttgcttga tgaaatagga catatcaaat taacagattt tggactcagc
661 aaggagtcag tagatcaaga aaagaaggct tactcatttt gtggtacagt agagtatatg
721 gctcctgaag tagtaaatag gagaggccat tcccagagtg ctgattggtg gtcatatggt
781 gttcttatgt ttgaaatgct tactggtact ctgccatttc aaggtaaaga cagaaatgag
841 accatgaata tgatattaaa agcaaaactt ggaatgcctc aacttcttag tgctgaagca
901 caaagtcttc taaggatgtt attcaaaagg aatccagcaa acagattggg atcagaagga
961 gttgaagaaa tcaaaagaca tctgtttttt gcaaatattg actgggataa attatataaa
1021 ggagaagttc aacctccttt caaacctgct tctggaaaac cagatgatac tttttgtttt
1081 gatcctgaat ttactgcaaa aacacctaaa gattctcccg gtttgccagc cagtgcaaat
1141 gctcatcagc tcttcaaagg attcagcttt gttgcaactt ctattgcaga agaatataaa
1201 atcactccta tcacaagtgc aaatgtatta ccaattgttc agataaatgg aaatgctgca
1261 caatttggtg aagtatatga attgaaggag gatattggtg ttggctccta ctctgtttgc
1321 aagcgatgca tacatgcaac taccaacatg gaatttgcag tgaagatcat tgacaaaagt
1381 aagcgagacc cttcagagga gattgaaata ttgatgcgct atggacaaca tcccaacatt
1441 attactttga aggatgtctt tgatgatggt agatatgttt accttgttac ggatttaatg
1501 aaaggaggag agttacttga ccgtattctc aaacaaaaat gtttctcgga acgggaggct
1561 agtgatatac tatatgtaat aagtaagaca gttgactatc ttcattgtca aggagttgtt
1621 catcgtgatc ttaaacctag taatatttta tacatggatg aatcagccag tgcagattca
1681 atcaggatat gtgattttgg gtttgcaaaa caacttcgag gagaaaatgg acttctctta
1741 actccatgct acactgcaaa ctttgttgca cccgaggttc ttatgcaaca gggatatgat
1801 gctgcttgtg atatctggag tttaggagtc cttttttaca caatgttggc tggctacact
1861 ccatttgcta atggccccaa tgatactcct gaagagatac tgctgcgtat aggcaatgga
1921 aaattctctt tgagtggtgg aaactgggac aatatttcag acggagcaaa ggatttgctt
1981 tcccatatgc ttcatatgga cccacatcag cggtatactg ctgaacaaat attaaagcac
2041 tcatggataa ctcacagaga ccagttgcca aatgatcagc caaagagaaa tgatgtgtca
2101 catgttgtta agggagcaat ggttgcaaca tactctgccc tgactcacaa gacctttcaa
2161 ccagtcctag agcctgtagc tgcttcaagc ttagcccagc gacggagcat gaaaaagcga
2221 acatcaactg gcctgtaa
//