LOCUS CR536564 1200 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1022D for
gene MAP2K4, mitogen-activated protein kinase kinase 4; complete
cds, incl. stopcodon.
ACCESSION CR536564
VERSION CR536564.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1200)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1200)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E1022D, ORFNo 3171
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1022D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_003010 (gi24497520)
we found AA exchange(s) at position (first base of changed
triplet):
352(lys->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1200
/db_xref="H-InvDB:HIT000268500"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E1022D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1200
/codon_start=1
/gene="MAP2K4"
/db_xref="GOA:P45985"
/db_xref="H-InvDB:HIT000268500.13"
/db_xref="HGNC:HGNC:6844"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR008271"
/db_xref="InterPro:IPR011009"
/db_xref="InterPro:IPR017441"
/db_xref="PDB:3ALN"
/db_xref="PDB:3ALO"
/db_xref="PDB:3VUT"
/db_xref="UniProtKB/Swiss-Prot:P45985"
/protein_id="CAG38801.1"
/translation="MAAPSPSGGGGSGGGSGSGTPGPVGSPAPGHPAVSSMQGKRKAL
KLNFANPPFKSTARFTLNPNPTGVQNPHIERLRTHSIESSGKLKISPEQHWDFTAEDL
KDLGEIGRGAYGSVNRMVHKPSGQIMAVKRIRSTVDEKEQKQLLMDLDVVMRSSDCPY
IVQFYGALFREGDCWICMELMSTSFDKFYKYVYSVLDDVIPEEILGKITLATVKALNH
LKENLKIIHRDIKPSNILLDRSGNIKLCDFGISGQLVDSIAKTRDAGCRPYMAPERID
PSASRQGYDVRSDVWSLGITLYELATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEER
EFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATP
SSPMYVD"
BASE COUNT 362 a 259 c 284 g 295 t
ORIGIN
1 atggcggctc cgagcccgag cggcggcggc ggctccgggg gcggcagcgg cagcggcacc
61 cccggccccg tagggtcccc ggcgccaggc cacccggccg tcagcagcat gcagggtaaa
121 cgcaaagcac tgaagttgaa ttttgcaaat ccacctttca aatctacagc aaggtttact
181 ctgaatccca atcctacagg agttcaaaac ccacacatag agagactgag aacacacagc
241 attgagtcat caggaaaact gaagatctcc cctgaacaac actgggattt cactgcagag
301 gacttgaaag accttggaga aattggacga ggagcttatg gttctgtcaa cagaatggtc
361 cacaaaccaa gtgggcaaat aatggcagtt aaaagaattc ggtcaacagt ggatgaaaaa
421 gaacaaaaac aacttcttat ggatttggat gtagtaatgc ggagtagtga ttgcccatac
481 attgttcagt tttatggtgc actcttcaga gagggtgact gttggatctg tatggaactc
541 atgtctacct cgtttgataa gttttacaaa tatgtatata gtgtattaga tgatgttatt
601 ccagaagaaa ttttaggcaa aatcacttta gcaactgtga aagcactaaa ccacttaaaa
661 gaaaacttga aaattattca cagagatatc aaaccttcca atattcttct ggacagaagt
721 ggaaatatta agctctgtga cttcggcatc agtggacagc ttgtggactc tattgccaag
781 acaagagatg ctggctgtag gccatacatg gcacctgaaa gaatagaccc aagcgcatca
841 cgacaaggat atgatgtccg ctctgatgtc tggagtttgg ggatcacatt gtatgagttg
901 gccacaggcc gatttcctta tccaaagtgg aatagtgtat ttgatcaact aacacaagtc
961 gtgaaaggag atcctccgca gctgagtaat tctgaggaaa gggaattctc cccgagtttc
1021 atcaactttg tcaacttgtg ccttacgaag gatgaatcca aaaggccaaa gtataaagag
1081 cttctgaaac atccctttat tttgatgtat gaagaacgtg ccgttgaggt cgcatgctat
1141 gtttgtaaaa tcctggatca aatgccagct actcccagct ctcccatgta tgtcgattga
//