LOCUS CR536563 1191 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1022D for
gene CKN1, Cockayne syndrome 1 (classical); complete cds, incl.
stopcodon.
ACCESSION CR536563
VERSION CR536563.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1191)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1191)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1022D, ORFNo 3170
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1022D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_000082 (gi4557466)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1191
/db_xref="H-InvDB:HIT000268499"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1022D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1191
/codon_start=1
/gene="CKN1"
/db_xref="GOA:Q13216"
/db_xref="H-InvDB:HIT000268499.13"
/db_xref="HGNC:HGNC:3439"
/db_xref="InterPro:IPR001680"
/db_xref="InterPro:IPR015943"
/db_xref="InterPro:IPR017986"
/db_xref="InterPro:IPR019775"
/db_xref="InterPro:IPR020472"
/db_xref="InterPro:IPR036322"
/db_xref="InterPro:IPR042238"
/db_xref="PDB:4A11"
/db_xref="PDB:6FCV"
/db_xref="UniProtKB/Swiss-Prot:Q13216"
/protein_id="CAG38800.1"
/translation="MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGG
GINTLDIEPVEGRYMLSGGSDGVIVLYDLENSSRQSYYTCKAVCSIGRDHPDVHRYSV
ETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNFEETVYSHHMSPVSTKHCLVA
VGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSRVKLWDVRR
ASGCLITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSN
GENTLVNYGKVCNNSKKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHY
KTVDCCVFQSNFQELYSGSRDCNILAWVPSLYEPVPDDDETTTKSQLNPAFEDAWSSS
DEEG"
BASE COUNT 366 a 215 c 271 g 339 t
ORIGIN
1 atgctggggt ttttgtccgc acgccaaacg ggtttggagg accctcttcg ccttcggaga
61 gcagagtcaa cacggagagt tttgggactg gaattaaata aagacagaga tgttgaaaga
121 atccacggcg gtggaattaa cacccttgac attgaacctg ttgaagggag atacatgtta
181 tcaggtggtt cagatggtgt gattgtactt tatgaccttg agaactccag cagacaatct
241 tattacacat gtaaagcagt gtgttccatt ggcagagatc atcctgatgt tcacagatac
301 agtgtggaga ctgtacagtg gtatcctcat gacactggca tgttcacatc aagctcattt
361 gataaaactc tgaaagtatg ggatacaaat acattacaaa ctgcagatgt atttaatttt
421 gaggaaacag tttatagtca tcatatgtct ccagtctcca ccaagcactg tttggtagca
481 gttggtacta gaggacccaa agtacaactt tgtgacttga agtctggatc ctgttctcac
541 attctacagg gtcacagaca agaaatatta gcagtttcct ggtctccacg ttatgactat
601 atcttggcaa cagcaagtgc tgacagtaga gtaaaattat gggatgtgag aagagcatca
661 ggatgtttga ttactcttga tcaacataat gggaaaaagt cacaagctgt tgaatcagca
721 aacactgctc ataatgggaa agttaatggc ttatgtttta caagtgatgg acttcacctc
781 ctcactgttg gtacagataa tcgaatgagg ctctggaata gttccaatgg agaaaacaca
841 cttgtgaact atggaaaagt ttgtaataac agtaaaaaag gattgaaatt cactgtctcc
901 tgtggctgca gttcagaatt tgtttttgta ccatatggta gcaccattgc tgtttataca
961 gtttactcag gagaacagat aactatgctt aagggacatt ataaaactgt tgactgctgt
1021 gtatttcagt caaatttcca ggaactttat agtggtagca gagactgcaa cattctggct
1081 tgggttccat ccttatatga accagttcct gatgatgatg agactacaac aaaatcacaa
1141 ttaaatccgg cctttgaaga tgcctggagc agcagtgatg aagaaggatg a
//