LOCUS CR536562 1143 bp mRNA linear HUM 23-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H1220D for
gene FEN1, flap structure-specific endonuclease 1; complete cds,
incl. stopcodon.
ACCESSION CR536562
VERSION CR536562.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1143)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1143)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H1220D, ORFNo 3169
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H1220D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_004111 (gi19718776)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1143
/db_xref="H-InvDB:HIT000268498"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H1220D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1143
/codon_start=1
/gene="FEN1"
/db_xref="GOA:Q6FHX6"
/db_xref="H-InvDB:HIT000268498.14"
/db_xref="InterPro:IPR006084"
/db_xref="InterPro:IPR006085"
/db_xref="InterPro:IPR006086"
/db_xref="InterPro:IPR008918"
/db_xref="InterPro:IPR019974"
/db_xref="InterPro:IPR023426"
/db_xref="InterPro:IPR029060"
/db_xref="InterPro:IPR036279"
/db_xref="UniProtKB/TrEMBL:Q6FHX6"
/protein_id="CAG38799.1"
/translation="MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLI
AVRQGGDVLQNEEGETTSHLMGMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSE
RRAEAEKQLQQAQAAGAEQEVEKFTKRLVKVTKQHNDECKHLLSLMGIPYLDAPSEAE
ASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLTASEAKKLPIQEFHLSRILQELGL
NQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIVRRLDPNKYPVPENWLH
KEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGVKRLSKSRQG
STQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGK"
BASE COUNT 291 a 283 c 353 g 216 t
ORIGIN
1 atgggaattc aaggcctggc caaactaatt gctgatgtgg cccccagtgc catccgggag
61 aatgacatca agagctactt tggccgtaag gtggccattg atgcctctat gagcatttat
121 cagttcctga ttgctgttcg ccagggtggg gatgtgctgc agaatgagga gggtgagacc
181 accagccacc tgatgggcat gttctaccgc accattcgca tgatggagaa cggcatcaag
241 cccgtgtatg tctttgatgg caagccgcca cagctcaagt caggcgagct ggccaaacgc
301 agtgagcggc gggctgaggc agagaagcag ctgcagcagg ctcaggctgc tggggccgag
361 caggaggtgg aaaaattcac taagcggctg gtgaaggtca ctaagcagca caatgatgag
421 tgcaaacatc tgctgagcct catgggcatc ccttatcttg atgcacccag tgaggcagag
481 gccagctgtg ctgccctggt gaaggctggc aaagtctatg ctgcggctac cgaggacatg
541 gactgcctca ccttcggcag ccctgtgcta atgcgacacc tgactgccag tgaagccaaa
601 aagctgccaa tccaggaatt ccacctgagc cggattctgc aggagctggg cctgaaccag
661 gaacagtttg tggatctgtg catcctgcta ggcagtgact actgtgagag tatccggggt
721 attgggccca agcgggctgt ggacctcatc cagaagcaca agagcatcga ggagatcgtg
781 cggcgacttg accccaacaa gtaccctgtg ccagaaaatt ggctccacaa ggaggctcac
841 cagctcttct tggaacctga ggtgctggac ccagagtctg tggagctgaa gtggagcgag
901 ccaaatgaag aagagctgat caagttcatg tgtggtgaaa agcagttctc tgaggagcga
961 atccgcagtg gggtcaagag gctgagtaag agccgccaag gcagcaccca gggccgcctg
1021 gatgatttct tcaaggtgac cggctcactc tcttcagcta agcgcaagga gccagaaccc
1081 aagggatcca ctaagaagaa ggcaaagact ggggcagcag ggaagtttaa aaggggaaaa
1141 taa
//