LOCUS CR536548 1017 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0920D for
gene MDH2, malate dehydrogenase 2, NAD (mitochondrial); complete
cds, incl. stopcodon.
ACCESSION CR536548
VERSION CR536548.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1017)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1017)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0920D, ORFNo 3145
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0920D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence BC001917
we found AA exchange(s) at position (first base of changed
triplet):
16(ala->val)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1017
/db_xref="H-InvDB:HIT000268484"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0920D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1017
/codon_start=1
/gene="MDH2"
/db_xref="GOA:Q6FHZ0"
/db_xref="H-InvDB:HIT000268484.13"
/db_xref="InterPro:IPR001236"
/db_xref="InterPro:IPR001252"
/db_xref="InterPro:IPR001557"
/db_xref="InterPro:IPR010097"
/db_xref="InterPro:IPR015955"
/db_xref="InterPro:IPR022383"
/db_xref="InterPro:IPR036291"
/db_xref="UniProtKB/TrEMBL:Q6FHZ0"
/protein_id="CAG38785.1"
/translation="MLSALVRPVSAALRRSFSTSAQNNAKVAVLGASGGIGQPLSLLL
KNSPLVSRLTLYDIAHTPGVAADLSHIETKAAVKGYLGPEQLPDCLKGCDVVVIPAGV
PRKPGMTRDDLFNTNATIVATLTAACAQHCPEAMICVIANPVNSTIPITAEVFKKHGV
YNPNKIFGVTTLDIVRANTFVAELKGLDPARVNVPVIGGHAGKTIIPLISQCTPKVDF
PQDQLTALTGRIQEAGTEVVKAKAGAGSATLSMAYAGARFVFSLVDAMNGKEGVVECS
FVKSQETECTYFSTPLLLGKKGIEKNLGIGKVSSFEEKMISDAIPELKASIKKGEDFV
KTLK"
BASE COUNT 230 a 308 c 272 g 207 t
ORIGIN
1 atgctctccg ccctcgtccg gcctgtcagc gctgctctcc gccgcagctt cagcacctcg
61 gcccagaaca atgctaaagt agctgtgcta ggggcctctg gaggcatcgg gcagccactt
121 tcacttctcc tgaagaacag ccccttggtg agccgcctga ccctctatga tatcgcgcac
181 acacccggag tggccgcaga tctgagccac atcgagacca aagccgctgt gaaaggctac
241 ctcggacctg aacagctgcc tgactgcctg aaaggttgtg atgtggtagt tattccggct
301 ggagtcccca gaaagccagg catgacccgg gacgacctgt tcaacaccaa tgccacgatt
361 gtggccaccc tgaccgctgc ctgtgcccag cactgcccgg aagccatgat ctgcgtcatt
421 gccaatccgg ttaattccac catccccatc acagcagaag ttttcaagaa gcatggagtg
481 tacaacccca acaaaatctt cggcgtgacg accctggaca tcgtcagagc caacaccttt
541 gttgcagagc tgaagggttt ggatccagct cgagtcaacg tccctgtcat tggtggccat
601 gctgggaaga ccatcatccc cctgatctct cagtgcaccc ccaaggtgga ctttccccag
661 gaccagctga cagcactcac tgggcggatc caggaggccg gcacggaggt ggtcaaggct
721 aaagccggag caggctctgc caccctctcc atggcgtatg ccggcgcccg ctttgtcttc
781 tcccttgtgg atgcaatgaa tggaaaggaa ggtgttgtgg aatgttcctt cgttaagtca
841 caggaaacgg aatgtaccta cttctccaca ccgctgctgc ttgggaaaaa gggcatcgag
901 aagaacctgg gcatcggcaa agtctcctct tttgaggaga agatgatctc ggatgccatc
961 cccgagctga aggcctccat caagaagggg gaagatttcg tgaagaccct gaagtga
//