LOCUS       CR536534                 483 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834C0622D for
            gene MBP, myelin basic protein; complete cds, incl. stopcodon.
ACCESSION   CR536534
VERSION     CR536534.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 483)
  AUTHORS   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
            Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
            Korn B., Zuo D., Hu Y., LaBaer J.
  JOURNAL   Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834C0622D, ORFNo 3114
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0622D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full ORF clones
            jointly established and verified by the Harvard Institute of
            Proteomics and RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC TCC ACC (ATG).
            The stopcodon is followed by the 3' att site:
            (stop)GACCCAGCTTTCTT..att
            Compared to the reference sequence M30047
            we found AA exchange(s) at position (first base of changed
            triplet):
            466(pro->thr)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..483
                     /db_xref="H-InvDB:HIT000268470"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834C0622D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..483
                     /codon_start=1
                     /gene="MBP"
                     /db_xref="GOA:P02686"
                     /db_xref="H-InvDB:HIT000268470.12"
                     /db_xref="HGNC:HGNC:6925"
                     /db_xref="InterPro:IPR000548"
                     /db_xref="PDB:1BX2"
                     /db_xref="PDB:1FV1"
                     /db_xref="PDB:1HQR"
                     /db_xref="PDB:1K2D"
                     /db_xref="PDB:1QCL"
                     /db_xref="PDB:1YMM"
                     /db_xref="PDB:1ZGL"
                     /db_xref="UniProtKB/Swiss-Prot:P02686"
                     /protein_id="CAG38771.1"
                     /translation="MASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGR
                     FFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPP
                     SQGKGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSTMARR
                     "
BASE COUNT          123 a          148 c          136 g           76 t
ORIGIN      
        1 atggcgtcac agaagagacc ctcccagagg cacggatcca agtacctggc cacagcaagt
       61 accatggacc atgccaggca tggcttcctc ccaaggcaca gagacacggg catccttgac
      121 tccatcgggc gcttctttgg cggtgacagg ggtgcgccca agcggggctc tggcaaggac
      181 tcacaccacc cggcaagaac tgctcactac ggctccctgc cccagaagtc acacggccgg
      241 acccaagatg aaaaccccgt agtccacttc ttcaagaaca ttgtgacgcc tcgcacacca
      301 cccccgtcgc agggaaaggg ggccgaaggc cagagaccag gatttggcta cggaggcaga
      361 gcgtccgact ataaatcggc tcacaaggga ttcaagggag tcgatgccca gggcacgctt
      421 tccaaaattt ttaagctggg aggaagagat agtcgctctg gatcaaccat ggctagacgc
      481 tga
//