LOCUS CR536534 483 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C0622D for gene MBP, myelin basic protein; complete cds, incl. stopcodon. ACCESSION CR536534 VERSION CR536534.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 483) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 483) AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834C0622D, ORFNo 3114 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0622D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full ORF clones jointly established and verified by the Harvard Institute of Proteomics and RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC TCC ACC (ATG). The stopcodon is followed by the 3' att site: (stop)GACCCAGCTTTCTT..att Compared to the reference sequence M30047 we found AA exchange(s) at position (first base of changed triplet): 466(pro->thr) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..483 /db_xref="H-InvDB:HIT000268470" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834C0622D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..483 /codon_start=1 /gene="MBP" /db_xref="GOA:P02686" /db_xref="H-InvDB:HIT000268470.12" /db_xref="HGNC:HGNC:6925" /db_xref="InterPro:IPR000548" /db_xref="PDB:1BX2" /db_xref="PDB:1FV1" /db_xref="PDB:1HQR" /db_xref="PDB:1K2D" /db_xref="PDB:1QCL" /db_xref="PDB:1YMM" /db_xref="PDB:1ZGL" /db_xref="UniProtKB/Swiss-Prot:P02686" /protein_id="CAG38771.1" /translation="MASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGR FFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPP SQGKGAEGQRPGFGYGGRASDYKSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSTMARR " BASE COUNT 123 a 148 c 136 g 76 t ORIGIN 1 atggcgtcac agaagagacc ctcccagagg cacggatcca agtacctggc cacagcaagt 61 accatggacc atgccaggca tggcttcctc ccaaggcaca gagacacggg catccttgac 121 tccatcgggc gcttctttgg cggtgacagg ggtgcgccca agcggggctc tggcaaggac 181 tcacaccacc cggcaagaac tgctcactac ggctccctgc cccagaagtc acacggccgg 241 acccaagatg aaaaccccgt agtccacttc ttcaagaaca ttgtgacgcc tcgcacacca 301 cccccgtcgc agggaaaggg ggccgaaggc cagagaccag gatttggcta cggaggcaga 361 gcgtccgact ataaatcggc tcacaaggga ttcaagggag tcgatgccca gggcacgctt 421 tccaaaattt ttaagctggg aggaagagat agtcgctctg gatcaaccat ggctagacgc 481 tga //