LOCUS CR536496 666 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1117D for
gene RAB27A, RAB27A, member RAS oncogene family; complete cds,
incl. stopcodon.
ACCESSION CR536496
VERSION CR536496.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 666)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 666)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E1117D, ORFNo 3048
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1117D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence NM_183235 (gi34485708)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..666
/db_xref="H-InvDB:HIT000268434"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E1117D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..666
/codon_start=1
/gene="RAB27A"
/db_xref="GOA:P51159"
/db_xref="H-InvDB:HIT000268434.12"
/db_xref="HGNC:HGNC:9766"
/db_xref="InterPro:IPR001806"
/db_xref="InterPro:IPR005225"
/db_xref="InterPro:IPR027417"
/db_xref="InterPro:IPR041837"
/db_xref="PDB:6HUF"
/db_xref="UniProtKB/Swiss-Prot:P51159"
/protein_id="CAG38735.1"
/translation="MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGI
DFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTAFFRDAMGFLLLFDLT
NEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYF
ETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGA
CGC"
BASE COUNT 205 a 116 c 185 g 160 t
ORIGIN
1 atgtctgatg gagattatga ttacctcatc aagtttttag ctttgggaga ctctggtgta
61 gggaagacca gtgtacttta ccaatataca gatggtaaat ttaactccaa atttatcaca
121 acagtgggca ttgatttcag ggaaaaaaga gtggtgtaca gagccagtgg gccggatgga
181 gccactggca gaggccagag aatccacctg cagttatggg acacagcagg gcaggagagg
241 tttcgtagct taacgacagc gttcttcaga gatgctatgg gttttcttct actttttgat
301 ctgacaaatg agcaaagttt cctcaatgtc agaaactgga taagccagct acagatgcat
361 gcatattgtg aaaacccaga tatagtgctg tgtggaaaca agagtgatct ggaggaccag
421 agagtagtga aagaggagga agccatagca ctcgcagaga aatatggaat cccctacttt
481 gaaactagtg ctgccaatgg gacaaacata agccaagcaa ttgagatgct tctggacctg
541 ataatgaagc gaatggaacg gtgtgtggac aagtcctgga ttcctgaagg agtggtgcga
601 tcaaatggtc atgcctctac ggatcagtta agtgaagaaa aggagaaagg ggcatgtggc
661 tgttga
//