LOCUS CR536491 1125 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0420D for
gene ADH5, alcohol dehydrogenase 5 (class III), chi polypeptide;
complete cds, incl. stopcodon.
ACCESSION CR536491
VERSION CR536491.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1125)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1125)
AUTHORS Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
Korn B., Zuo D., Hu Y., LaBaer J.
JOURNAL Submitted (23-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0420D, ORFNo 3039
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0420D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
jointly established and verified by the Harvard Institute of
Proteomics and RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC TCC ACC (ATG).
The stopcodon is followed by the 3' att site:
(stop)GACCCAGCTTTCTT..att
Compared to the reference sequence BC014665
we found AA exchange(s) at position (first base of changed
triplet):
592(phe->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1125
/db_xref="H-InvDB:HIT000268429"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0420D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1125
/codon_start=1
/gene="ADH5"
/db_xref="GOA:Q6FI45"
/db_xref="H-InvDB:HIT000268429.12"
/db_xref="InterPro:IPR002328"
/db_xref="InterPro:IPR011032"
/db_xref="InterPro:IPR013149"
/db_xref="InterPro:IPR013154"
/db_xref="InterPro:IPR014183"
/db_xref="InterPro:IPR036291"
/db_xref="UniProtKB/TrEMBL:Q6FI45"
/protein_id="CAG38730.1"
/translation="MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAV
CHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKF
CLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKI
DPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVIGLGGVGLAVIMGCKVAGASR
IIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMR
AALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSE
YMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI"
BASE COUNT 307 a 209 c 306 g 303 t
ORIGIN
1 atggcgaacg aggttatcaa gtgcaaggct gcagttgctt gggaggctgg aaagcctctc
61 tccatagagg agatagaggt ggcaccccca aaggctcatg aagttcgaat caagatcatt
121 gccactgcgg tttgccacac cgatgcctat accctgagtg gagctgatcc tgagggttgt
181 tttccagtga tcttgggaca tgaaggtgct ggaattgtgg aaagtgttgg tgagggagtt
241 actaagctga aggcgggtga cactgtcatc ccactttaca tcccacagtg tggagaatgc
301 aaattttgtc taaatcctaa aactaacctt tgccagaaga taagagtcac tcaagggaaa
361 ggattaatgc cagatggtac cagcagattt acttgcaaag gaaagacaat tttgcattac
421 atgggaacca gcacattttc tgaatacaca gttgtggctg atatctctgt tgctaaaata
481 gatcctttag cacctttgga taaagtctgc cttctaggtt gtggcatttc aaccggttat
541 ggtgctgctg tgaacactgc caagttggag cctggctctg tttgtgccgt cattggtctg
601 ggaggagtcg gattggcagt tatcatgggc tgtaaagtgg ctggtgcttc ccggatcatt
661 ggtgtggaca tcaataaaga taaatttgca agggccaaag agtttggagc cactgaatgt
721 attaaccctc aggattttag taaacccatc caggaagtgc tcattgagat gaccgatgga
781 ggagtggact attcctttga atgtattggt aatgtgaagg tcatgagagc agcacttgag
841 gcatgtcaca agggctgggg cgtcagcgtc gtggttggag tagctgcttc aggtgaagaa
901 attgccactc gtccattcca gctggtaaca ggtcgcacat ggaaaggcac tgcctttgga
961 ggatggaaga gtgtagaaag tgtcccaaag ttggtgtctg aatatatgtc caaaaagata
1021 aaagttgatg aatttgtgac tcacaatctg tcttttgatg aaatcaacaa agcctttgaa
1081 ctgatgcatt ctggaaagag cattcgaact gttgtaaaga tttaa
//