LOCUS CR533555 1738 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0521D for
gene DKFZP434K1421, hypothetical protein DKFZp434K1421; complete
cds, incl. stopcodon.
ACCESSION CR533555
VERSION CR533555.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1677)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1677)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0521D, ORFNo 2942
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0521D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence NM_032141 (gi14149806)
we found AA exchange(s) at position (first base of changed
triplet):
808(glu->gly)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1738
/db_xref="H-InvDB:HIT000268403"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0521D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1677
/codon_start=1
/gene="DKFZP434K1421"
/db_xref="GOA:Q9H0G5"
/db_xref="H-InvDB:HIT000268403.12"
/db_xref="HGNC:HGNC:25305"
/db_xref="InterPro:IPR018612"
/db_xref="UniProtKB/Swiss-Prot:Q9H0G5"
/protein_id="CAG38586.1"
/translation="MAIPGRQYGLILPKKTQQLHPVLQKPSVFGNDSDDDDETSVSES
LQREAAKKQAMKQTKLEIQKALAEDATVYEYDSIYDEMQKKKEENNPKLLLGKDRKPK
YIHNLLKAVEIRKKEQEKRMEKKIQREREMEKGEFDDKEAFVTSAYKKKLQERAEEEE
REKRAAALEACLDVTKQKDLSGFYRHLLNQAVGEEEVPKCSFREARSGIKEEKSRGFS
NEVSSKNRIPQEKCILQTDVKVEENPDADSDFDAKSSADDEIEETRVNCRRGKVIETP
ENDFKHHRSQNHSRSPSEERGHSTRHHTKGSRTSRGHEKREDQHQQKQSRDQENHYTD
RDYRKERDSHRHREASHRDSHWKRHEQEDKPRARDQRERSDRVWKREKDREKYSQREQ
ERDRQQNDQNRPSEKGEKEEKSKAKEEHMKVRKERYENNDKYRDREKREVGVQSSERN
QDRKESSPNSRAKDKFLDQERSNKMRNMAKDKERNQEKPSNSESSLGAKHRLTEEGQE
KGKEQERPPEAVSKFAKRNNEETVMSARDRYLARQMARVNAKTYIEKEDD"
BASE COUNT 718 a 280 c 455 g 285 t
ORIGIN
1 atggcgattc cgggcaggca gtatgggctt attttgccaa agaaaacaca gcagttgcac
61 cctgttttgc aaaaaccatc agtgtttggg aatgattctg atgatgatga tgagacctct
121 gtgagtgaaa gccttcagag ggaagctgct aagaagcagg ccatgaaaca gaccaaactg
181 gaaatccaga aggcccttgc agaagatgct actgtgtatg aatatgacag tatttatgat
241 gaaatgcaga aaaaaaagga ggaaaataat cccaaattgc ttttggggaa agacagaaag
301 cccaagtata ttcacaactt gctaaaagca gttgagatca gaaaaaagga acaggaaaaa
361 agaatggaaa agaaaataca gagagaacga gaaatggaaa agggggagtt tgatgataaa
421 gaagcatttg tgacatctgc atataagaaa aaactgcaag agagagctga agaagaagaa
481 agagaaaaga gggctgctgc actggaagca tgtttggatg taaccaagca gaaagatctc
541 agtggatttt ataggcacct attaaatcaa gcagttggtg aagaggaagt acctaaatgc
601 agctttcgtg aagccagatc tggtataaag gaagaaaaat caaggggctt ctccaatgaa
661 gtaagttcaa aaaacagaat accacaagag aaatgcattc ttcaaactga tgtgaaagta
721 gaggaaaacc cagatgcaga cagtgacttc gatgctaaga gcagtgcgga tgatgaaata
781 gaagaaacta gagtgaactg cagaagggga aaggtcatag agacccctga gaatgacttc
841 aagcaccaca ggagtcaaaa ccactctcgg tcacctagtg aagaaagagg gcacagtacc
901 aggcaccaca cgaaaggatc acgaacgtcg agaggacatg agaaaaggga agatcagcac
961 cagcagaagc aatccagaga ccaagagaac cattacactg accgtgatta ccggaaagaa
1021 agggattctc ataggcacag agaggccagt catagagatt cccattggaa gaggcatgaa
1081 caggaagata aaccaagggc gagggaccaa agagaaagaa gtgacagagt atggaaaagg
1141 gagaaagata gggagaaata ttcccaaaga gaacaagaaa gagatagaca acaaaatgat
1201 cagaaccgac ccagtgagaa aggagagaag gaagagaaaa gcaaagcaaa ggaagagcat
1261 atgaaagtaa ggaaggaaag atatgaaaat aatgataaat acagagatag agaaaaacga
1321 gaggtaggtg ttcagtcttc agaaagaaat caagacagaa aggaaagcag cccaaattct
1381 agggcaaagg ataaatttct tgaccaagaa agatccaaca aaatgagaaa catggcaaag
1441 gacaaagaaa gaaaccaaga gaaaccctct aattctgaat catcactggg agcaaaacac
1501 agactcacag aggaagggca agagaagggt aaagaacaag agagaccacc tgaggcagtg
1561 agcaagtttg caaagcggaa caatgaagaa actgtaatgt cagctagaga caggtacttg
1621 gccaggcaga tggcgcgggt taatgcaaag acctatattg agaaagaaga tgattgatgg
1681 ctaccccaag agaaagattt aaggaagcac agaaaactgt aattcctgac ctgctgcg
//