LOCUS CR533553 2465 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G1021D for
gene FLJ12716, FLJ12716 protein; complete cds, incl. stopcodon.
ACCESSION CR533553
VERSION CR533553.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2448)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 2448)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G1021D, ORFNo 2937
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G1021D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136752
we found AA exchange(s) at position (first base of changed
triplet):
1135(arg->lys)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..2465
/db_xref="H-InvDB:HIT000268401"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G1021D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..2448
/codon_start=1
/gene="FLJ12716"
/db_xref="GOA:Q7Z392"
/db_xref="H-InvDB:HIT000268401.13"
/db_xref="HGNC:HGNC:25751"
/db_xref="InterPro:IPR021773"
/db_xref="InterPro:IPR025876"
/db_xref="UniProtKB/Swiss-Prot:Q7Z392"
/protein_id="CAG38584.1"
/translation="MSKQFQAFGDLFDEAIKLGLTAIQTQNPGFYYQQAAYYAQERKQ
LAKTLCNHEASVMYPNPDPLETQTGVLDFYGQRSWRQGILSFDLSDPEKEKVGILAIQ
LKERNVVHSEIIITLLSNAVAQFKKYKCPRMKSHLMVQMGEEYYYAKDYTKALKLLDY
VMCDYRSEGWWTLLTSVLTTALKCSYLMAQLKDYITYSLELLGRASTLKDDQKSRIEK
NLINVLMNESPDPEPDCDILAVKTAQKLWADRISLAGSNIFTIGVQDFVPFVQCKAKF
HAPSFHVDVPVQFDIYLKADCPHPIRFSKLCVSFNNQEYNQFCVIEEASKANEVLENL
TQGKMCLVPGKTRKLLFKFVAKTEDVGKKIEITSVDLALGNETGKCVVLNWQGGGGDA
ASSQEALQAARSFKRRPKLPDNEVHWDSIIIQASTMIISRVPNISVHLLHEPPALTNE
MYCLVVTVQSHEKTQIRDVKLTAGLKPGQDANLTQKTHVTLHGPELCDESYPALLTDI
PVGDLHPGEQLEKMLYVRCGTVGSRMFLVYVSYLINTTVEEKEIVCKCHKDETVTIET
VFPFDVAVKFVSTKFEHLERVYADIPFLLMTDLLSASPWALTIVSSELQLAPSMTTVD
QLESQVDNVILQTGESASECFCLQCPSLGNIEGGVATGHYIISWKRTSAMENIPIITT
VITLPHVIVENIPLHVNADLPSFGRVRESLPVKYHLQNKTDLVQDVEISVEPSDAFMF
SGLKQIRLRILPGTEQEMLYNFYPLMAGYQQLPSLNINLLRFPNFTNQLLRRFIPTSI
FVKPQGRLMDDTSIAAA"
BASE COUNT 713 a 521 c 533 g 698 t
ORIGIN
1 atgtctaaac aattccaggc ctttggagat ttatttgatg aagctattaa gttagggtta
61 acagctattc aaactcagaa tcctggtttc tattaccagc aggcagcata ctatgcccag
121 gagcggaaac agcttgcaaa aaccctctgt aaccacgaag cttctgtaat gtatcccaat
181 cctgatccct tagaaacaca aacaggcgtt cttgactttt atggacaaag atcatggcga
241 caaggaatac taagttttga tctttctgat cctgaaaaag aaaaggtggg aattcttgcc
301 attcagctga aggagagaaa tgttgttcac tctgagataa tcataactct tctgagcaat
361 gctgttgcac agttcaagaa gtataagtgc ccgcgaatga aaagtcacct aatggttcag
421 atgggagagg aatattatta cgcaaaggat tataccaaag ctttgaagtt gctggattat
481 gtgatgtgtg attatcggag tgaaggatgg tggactctgc tcacttctgt attaactaca
541 gctctgaagt gctcctacct catggcccaa ttaaaggatt acattactta ctccctagaa
601 ctccttggta gagcttcaac tctgaaagat gaccagaagt ctcggataga aaagaacctc
661 ataaatgttt taatgaatga aagtcctgat ccagaacccg actgtgatat cttagctgtg
721 aaaactgctc agaagctgtg ggcagaccga atttctctgg ctggcagcaa tattttcaca
781 ataggagtac aggactttgt gccatttgtg cagtgcaaag ccaagtttca tgccccaagt
841 tttcatgttg atgttcctgt tcagtttgat atttatctga aggctgattg tccacatccc
901 attaggtttt ccaagctctg tgtcagcttt aataatcagg aatacaacca gttctgtgta
961 atagaagaag catccaaagc aaatgaagtt ttagaaaatc tgactcaagg aaagatgtgc
1021 ctagttcctg gcaaaacaag aaaactgtta tttaagtttg ttgcaaaaac tgaagatgtg
1081 ggaaagaaaa ttgagattac ttcagtggat cttgctctgg gcaatgagac gggaaaatgt
1141 gtggttttaa attggcaggg aggaggagga gatgctgctt cctcccaaga agccttacag
1201 gcagctcggt ctttcaaaag gcgacctaag ctacctgaca atgaagttca ctgggacagc
1261 attataattc aggcaagcac aatgatcata tccagagtcc caaacatttc tgtacatctg
1321 ctacatgaac cccctgcact gactaatgaa atgtattgtt tggttgtgac tgttcagtcc
1381 catgaaaaga cccaaatcag agatgtgaag ctcactgctg gcttaaaacc aggacaggat
1441 gccaatttaa ctcagaagac tcacgtgact cttcatggac cagaactgtg tgatgaatcc
1501 tacccggctt tactcactga cattcctgtt ggagacttac atccagggga acagctggaa
1561 aaaatgttgt atgttcgctg tggaacagtg ggttccagaa tgtttcttgt atatgtttct
1621 tacctgataa atacaaccgt tgaagaaaaa gaaattgttt gcaagtgtca caaggatgaa
1681 actgtaacaa ttgaaacagt ctttccattt gatgttgcgg ttaaatttgt ttctaccaag
1741 tttgagcacc tggaaagggt ttatgctgac atcccctttc tgttgatgac ggacctctta
1801 agtgcctcac cctgggccct cactattgtt tccagtgagc tccagcttgc tccatccatg
1861 accacagtgg accagctcga gtctcaagtg gacaatgtta tcttacagac tggagagagt
1921 gctagtgaat gcttttgtct tcaatgccca tctcttggaa atattgaagg tggagtagca
1981 accgggcatt atattatctc ttggaaaagg acctcagcaa tggagaatat ccccatcatc
2041 acaactgtca tcactctgcc gcacgtgatt gtggagaata tccctctcca tgtgaatgca
2101 gatctgccgt catttgggcg tgtcagagag tcgttacctg tcaagtatca cctacagaat
2161 aagaccgact tagttcaaga tgtagaaatt tctgtggagc ccagtgatgc cttcatgttc
2221 tcaggtctca aacagattcg attacgtatc ctccctggca cggagcagga aatgctatat
2281 aatttctatc ctctgatggc tggataccag cagctgccat ctctcaacat caacttgctt
2341 agatttccta acttcacaaa tcagctgctc aggcgtttta tacctaccag tatttttgtc
2401 aagccacagg gtcgactcat ggatgatacc tctattgctg ctgcatgatg ttcaagaccg
2461 gccct
//