LOCUS CR533550 1469 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0919D for
gene FLJ20297, hypothetical protein FLJ20297; complete cds, incl.
stopcodon.
ACCESSION CR533550
VERSION CR533550.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1287)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1287)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H0919D, ORFNo 2929
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0919D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136737
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1469
/db_xref="H-InvDB:HIT000268398"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H0919D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1287
/codon_start=1
/gene="FLJ20297"
/db_xref="GOA:Q9NXE4"
/db_xref="H-InvDB:HIT000268398.14"
/db_xref="HGNC:HGNC:32949"
/db_xref="InterPro:IPR024129"
/db_xref="UniProtKB/Swiss-Prot:Q9NXE4"
/protein_id="CAG38581.1"
/translation="MWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTK
LFVGFLNRALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQH
RLFTAPTFTGSFLSPWPPAVTDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQ
LITQAKHTAKSISDQCAESPAGHSFLSWLGFSSMDTNGSYTANDLDEMGQDSVRKTDE
YLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIVGEDGLILTPLGRYQ
IINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDDFL
GSFCRYHLTEPGLASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVAS
LFCVGPLPCTLLLTLGYVLYASAMTLLTERGKLHQP"
BASE COUNT 288 a 479 c 433 g 269 t
ORIGIN
1 atgtggctga gctacctgca gccgtggcgg tacgcgcctg acaagcaggc tccgggcagc
61 gactcccagc cccggtgtgt gtcggagaaa tgggcaccct ttgtccagga gaacctgctg
121 atgtacacca agttgtttgt gggctttctg aaccgcgcgc tccgcacaga cctggtcagc
181 cccaagcacg cgctcatggt gttccgagtg gccaaagtct ttgcccagcc caacctggct
241 gagatgattc agaaaggtga gcagctattc ctggagccag agctggtcat cccccaccgc
301 cagcaccgac tcttcacggc ccccacattc actgggagct tcctgtcacc ctggccacca
361 gcggtcactg atgcctcctt caaggtgaag agccacgtct acagcctgga gggccaggac
421 tgcaagtaca ccccgatgtt tgggcccgag gcccgcaccc tggtcctgcg cctcgctcag
481 ctcatcacac aggccaaaca cacagccaag tccatctccg accagtgtgc ggagagcccg
541 gctggccact ccttcctctc atggctgggc tttagctcca tggacaccaa tggctcctac
601 acagccaacg acctggacga gatggggcaa gacagtgtcc ggaagacaga tgaatacctg
661 gagaaggccc tggagtacct gcgccagata ttccggctca gcgaagcgca gctcaggcag
721 ttcacactcg ccttgggcac cacccaggat gagaatggaa aaaagcaact ccccgactgc
781 atcgtgggtg aggacggact catccttacg cccctggggc ggtaccagat catcaatggg
841 ctgcgaaggt ttgaaattga gtaccagggg gacccggagc tgcagcccat ccggagctat
901 gagatcgcca gcttggtccg cacactcttt aggctgtcgt ctgccatcaa ccacagattt
961 gcaggacaga tggcggctct gtgttcccgg gatgacttcc tcggcagctt ctgtcgctac
1021 cacctcacag aacctgggct ggccagcagg cacctgctga gccctgtggg gcggaggcag
1081 gtggccggcc acacccgcgg ccccaggctc agcctgcgct tcctgggcag ttaccggacg
1141 ctggtctcgc tgctgctggc cttcttcgtg gcctctctgt tctgcgtcgg gcccctccca
1201 tgcacgctgc tgctcaccct gggctatgtc ctctacgcct ctgccatgac actgctgacc
1261 gagcggggga agctgcacca gccctgaagg tgtcagctgc cttcagagca ggctggaggg
1321 atttgccaca cagccccacc cttgggctga gaggacctgg gaagcccctc caggagggaa
1381 cacggtcatc ctcgggcttc tggagcgggg ttcctgcagc cgcagaggca tctggaggaa
1441 acgcaaccaa gaaaggaagg caggtgggc
//