LOCUS CR533542 1184 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0719D for
gene DKFZP434G072, hypothetical protein DKFZp434G072; complete cds,
incl. stopcodon.
ACCESSION CR533542
VERSION CR533542.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1080)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1080)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0719D, ORFNo 2912
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0719D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence NM_032149 (gi14149820)
we found AA exchange(s) at position (first base of changed
triplet):
865(lys->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1184
/db_xref="H-InvDB:HIT000268390"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0719D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1080
/codon_start=1
/gene="DKFZP434G072"
/db_xref="H-InvDB:HIT000268390.13"
/db_xref="HGNC:HGNC:25274"
/db_xref="InterPro:IPR037394"
/db_xref="UniProtKB/Swiss-Prot:Q53FE4"
/protein_id="CAG38573.1"
/translation="MNLNPPTSALQIEGKGSHIMARNVSCFLVRHTPHPRRVCHIKGL
NNIPICTVNDDENAFGTLWEVGQSNYLEKNRIPFANCSYPPSTAVQKSPVRGMSPAPN
GAKVPPRPHSEPSRKIKECFKTSSENPLVIKKEEIKAKRPPSPPKACSTPGSCSSGMT
STKNDVKANTICIPNYLDQEIKILAKLCSILHTDSLAEVLQWLLHATSKEKEWVSALI
HSELAEINLLTHHRRNTSMEPAAETGKPPTVKSPPTVKLPPNFTAKSKVLTRDTEGDQ
PTRVSSQGSEENREVPKEAEHKPPLLIRRNNMKIPVAEYFSKPNSPPRPNTQESGSAK
PVSARSIQEYNLCPQRACYPSTHRR"
BASE COUNT 401 a 278 c 236 g 269 t
ORIGIN
1 atgaacctca accccccgac atctgctctt cagatcgagg gcaaaggcag ccatattatg
61 gctagaaatg taagctgctt tctagtcagg cacacccctc atcccagaag agtctgccac
121 atcaaaggct tgaataacat tccaatctgt actgtgaatg atgatgagaa tgcatttgga
181 acattgtggg aagttggcca gtctaactac ttagagaaga acaggatacc atttgccaat
241 tgcagttacc ccccgagcac tgcagtccag aagagccctg taagaggaat gtcgccagcc
301 ccaaacggtg ccaaagtgcc tccacggcct cattctgagc ccagtagaaa aattaaagag
361 tgcttcaaaa cttccagtga gaatccctta gtaattaaaa aggaagaaat taaggccaaa
421 agaccaccat cacctccaaa ggcatgctct actcctggct cctgttcttc agggatgaca
481 agtaccaaga atgatgtgaa agcaaacacc atttgcatac caaactatct ggatcaggaa
541 ataaaaatcc tggcaaagct ctgtagcatt ttgcatactg attctctggc agaagtttta
601 cagtggctgc ttcatgcaac ttcaaaagaa aaagagtggg tctcagcttt gattcattct
661 gagcttgccg agataaacct gttaactcat cacagaagaa acacctcaat ggaaccagca
721 gcagagactg ggaagccacc cacagttaaa tcaccaccca cagttaaatt gcccccaaat
781 tttactgcaa aatcaaaagt gctgaccaga gatacagaag gggatcaacc aaccagagtg
841 tcaagtcaag gatctgaaga aaacagggaa gtaccaaaag aggctgagca caagcctcca
901 ctacttataa gaagaaataa tatgaaaata cctgttgcag aatatttcag caaaccaaat
961 tctcctccca ggcctaacac tcaggagagt ggatcagcaa aaccagtgtc agcaaggagt
1021 atacaagaat acaacctctg tccccaaaga gcatgttatc cttcaacaca ccggaggtag
1081 aagttctaga ctgggtgaat tctttcatga atatgagctt cacatttaca tcatcaaatt
1141 atttttcaaa tgaatatttt tggtattgag gaatcaagtg gtcc
//