LOCUS CR533535 1392 bp mRNA linear HUM 22-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G0419D for
gene TSPYL, TSPY-like; complete cds, incl. stopcodon.
ACCESSION CR533535
VERSION CR533535.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1317)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1317)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G0419D, ORFNo 2892
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G0419D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136629
we found AA exchange(s) at position (first base of changed
triplet):
700(glu->lys)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1392
/db_xref="H-InvDB:HIT000268383"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G0419D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1317
/codon_start=1
/gene="TSPYL"
/db_xref="GOA:Q6FI91"
/db_xref="H-InvDB:HIT000268383.12"
/db_xref="InterPro:IPR002164"
/db_xref="InterPro:IPR037231"
/db_xref="UniProtKB/TrEMBL:Q6FI91"
/protein_id="CAG38566.1"
/translation="MSGLDGVKRTTPLQTHSIIISDQVPSDQDAHQYLRLRDQSEATQ
VMAEPGEGGSETVALPPSPPSEEGGVPQDPAGRGGTPQIRVVGGRGHVAIKAGQEEGQ
PPAEGLAAASVVMAADRSLKKGVQGGEKALEICGAQRSASELTAGAEAEAEEVKTGKC
ATVSAAVAERESAEVVVKEGLAEKEVMEEQMEVEEQPPEGEEIEVAEEDRLEEEAREE
EGPWPLHEALRMDPLKAIQLELDTVNAQADRAFQQLEHKFGRMRRHYLERRNYIIQNI
PGFWMTAFRNHPQLSAMIRGQDAEMLRYITNLEVKELRHPRTGCKFKFFFRRNPYFRN
KLIVKEYEVRSSGRVVSLSTPIIWRRGHEPQSFIRRNQDLICSFFTWFSDHSLPESDK
IAEIIKEDLWPNPLQYYLLREGVRRARRRPLREPVEIPRPFGFQSG"
BASE COUNT 333 a 361 c 441 g 257 t
ORIGIN
1 atgagcggcc tggatggggt caagaggacc actcccctcc aaacccacag catcattatt
61 tctgaccaag tcccgagcga ccaggacgca caccagtacc tgaggctccg cgaccaaagc
121 gaggcgacac aggtgatggc ggagccgggt gagggaggct cggagaccgt cgcgctcccg
181 ccttcaccgc cttcagagga ggggggcgta ccccaggatc ccgcgggccg tggcggtact
241 ccccagatcc gagttgttgg gggtcgcggt catgtggcga tcaaagccgg gcaggaagag
301 ggccagcctc ccgccgaagg cctggcagcc gcttctgtgg tgatggcagc cgaccgcagc
361 ctgaaaaagg gcgttcaggg tggagagaag gccctagaaa tctgtggcgc ccagagatcc
421 gcgtctgagc tgacggcggg ggcggaggct gaggcggagg aggtgaagac aggaaagtgc
481 gccaccgtct cagcagccgt ggctgagagg gagagcgctg aggtggtggt gaaggaaggc
541 ctggcggaga aggaggtaat ggaggagcag atggaggtag aggagcagcc gccagaaggt
601 gaagaaatag aagtggcgga ggaggataga ttggaggagg aggcgaggga ggaagaaggg
661 ccctggcctt tgcatgaggc tctccgcatg gaccctctga aggccatcca gctggaactg
721 gacactgtga atgctcaggc cgacagggcc ttccaacagc tggagcacaa gtttgggcgg
781 atgcgtcgac actacctgga gcggaggaac tacatcattc agaatatccc gggcttctgg
841 atgactgctt ttcgaaacca cccccagttg tccgccatga ttaggggcca agatgcagag
901 atgttaaggt acataaccaa tttagaggtg aaggaactca gacaccctag aaccggttgc
961 aagttcaagt tcttctttag aagaaacccc tacttcagaa acaagctgat tgtcaaggaa
1021 tatgaggtaa gatcctccgg ccgagtggtg tctctttcta ctccaattat atggcgcagg
1081 gggcatgaac cccagtcctt cattcgcaga aaccaagacc tcatctgcag cttcttcact
1141 tggttttcag accacagcct tccagagtcc gacaaaattg ctgagattat taaagaggat
1201 ctgtggccaa atccactgca atactacctg ttgcgtgaag gagtccgtag agcccgacgt
1261 cgcccgctaa gggagcctgt agagatcccc aggccctttg ggttccagtc tggttaacat
1321 ttgcccttgg gaatactcct gcacaaggtc tcctaccacc ttctgctgga cctgtgcttg
1381 ggcatcagca at
//