LOCUS CR533526 1105 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0119D for
gene HSPC142, HSPC142 protein; complete cds, incl. stopcodon.
ACCESSION CR533526
VERSION CR533526.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 990)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 990)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F0119D, ORFNo 2875
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0119D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136692
we found AA exchange(s) at position (first base of changed
triplet):
226(pro->ser) 862(ala->val) 904(pro->ser)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1105
/db_xref="H-InvDB:HIT000268374"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F0119D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..990
/codon_start=1
/gene="HSPC142"
/db_xref="GOA:Q9NWV8"
/db_xref="H-InvDB:HIT000268374.11"
/db_xref="HGNC:HGNC:25008"
/db_xref="InterPro:IPR026126"
/db_xref="InterPro:IPR036465"
/db_xref="PDB:6H3C"
/db_xref="UniProtKB/Swiss-Prot:Q9NWV8"
/protein_id="CAG38557.1"
/translation="MEVAEPSSPTEEEEEEEEHSAEPRPRTRSNPEGAEDRAVGAQAS
VGSRSEGEGEAASADDGSLNTSGAGPKSWQVSPPAPEVQIRTPRVNCPEKVIICLDLS
EEMSLPKLESFNGSKTNALNVSQKMIEMFVRTKHKIDKSHEFALVVVNDDTAWLSGLT
SDPRELCSCLYDLETASCSTFNLEGLFSLIQQKTELPVTENVQTIPPPYVVRTILVYS
RPPCQPQFSLTEPMKKMFQCPYFFFDVVYIHNGTEEKEEEMSWKDMFAFMGSLDTKGT
SYKYEVALAGPVLELHNCMAKLLAHSLQRPCQSHASYSLLEEEDEAIEVEATV"
BASE COUNT 251 a 308 c 325 g 221 t
ORIGIN
1 atggaagtgg cagagcccag cagccccact gaagaggagg aggaggaaga ggagcactcg
61 gcagagcctc ggccccgcac tcgctccaat cctgaagggg ctgaggaccg ggcagtaggg
121 gcacaggcca gcgtgggcag ccgcagcgag ggtgagggtg aggccgccag tgctgatgat
181 gggagcctca acacttcagg agccggccct aagtcctggc aggtgtcccc gccagcccct
241 gaggtccaaa ttcggacacc aagggtcaac tgtccagaga aagtgattat ctgcctggac
301 ctgtcagagg aaatgtcact gccaaagctg gagtcgttca acggctccaa aaccaacgcc
361 ctcaatgtct ctcagaagat gattgagatg ttcgtgcgga caaaacacaa gatcgacaaa
421 agccacgagt ttgcactggt ggtggtgaac gatgacacgg cctggctgtc tggcctgacc
481 tccgaccccc gcgagctctg tagctgcctc tatgatctgg agacggcctc ctgttccacc
541 ttcaatctgg aaggactttt cagcctcatc cagcagaaaa ctgagcttcc ggtcacagag
601 aacgtgcaga cgattccccc gccatatgtg gtccgcacca tccttgtcta cagccgtcca
661 ccttgccagc cccagttctc cttgacggag cccatgaaga aaatgttcca gtgcccatat
721 ttcttctttg acgttgttta catccacaat ggcactgagg agaaggagga ggagatgagt
781 tggaaggata tgtttgcctt catgggcagc ctggatacca agggtaccag ctacaagtat
841 gaggtggcac tggctgggcc agtcctggag ttgcacaact gcatggcgaa actgttggcc
901 cactccctgc agcggccttg ccagagccat gcttcctaca gcctgctgga ggaggaggat
961 gaagccattg aggttgaggc cactgtctga accatccctg tacatctgca ccttcttgtg
1021 caaggaagtc cttggcctaa agccttggtt ctcaaactgg gttccttggg acctccgggg
1081 tgggggggtt ccaggaggca cgtag
//