LOCUS CR533521 1301 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B1219D for
gene VMP1, likely ortholog of rat vacuole membrane protein 1;
complete cds, incl. stopcodon.
ACCESSION CR533521
VERSION CR533521.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1221)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1221)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B1219D, ORFNo 2867
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B1219D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136711
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1301
/db_xref="H-InvDB:HIT000268369"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B1219D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1221
/codon_start=1
/gene="VMP1"
/db_xref="GOA:Q96GC9"
/db_xref="H-InvDB:HIT000268369.11"
/db_xref="HGNC:HGNC:29559"
/db_xref="InterPro:IPR032816"
/db_xref="UniProtKB/Swiss-Prot:Q96GC9"
/protein_id="CAG38552.1"
/translation="MAENGKNCDQRRVAMNKEHHNGNFTDPSSVNEKKRREREERQNI
VLWRQPLITLQYFSLEILVILKEWTSKLWHRQSIVVSFLLLLAVLIATYYVEGVHQQY
VQRIEKQFLLYAYWIGLGILSSVGLGTGLHTFLLYLGPHIASVTLAAYECNSVNFPEP
PYPDQIICPDEEGTEGTIFLWSIISKVRIEACMWGIGTAIGELPPYFMARAARLSGAE
PDDEEYQEFEEMLEHAESAQDFASRAKLAVQKLVQKVGFFGILACASIPNPLFDLAGI
TCGHFLVPFWTFFGATLIGKAIIKMHIQKIFVIITFSKHIVEQMVAFIGAVPGIGPSL
QKPFQEYLEAQRQKLHHKSEMGTPQGENWLSWMFEKLVVVMVCYFILSIINSMAQSYA
KRIQQRLNSEEKTK"
BASE COUNT 378 a 249 c 313 g 361 t
ORIGIN
1 atggcagaga atggaaaaaa ttgtgaccag agacgtgtag caatgaacaa ggaacatcat
61 aatggaaatt tcacagaccc ctcttcagtg aatgaaaaga agaggaggga gcgggaagaa
121 aggcagaata ttgtcctgtg gagacagccg ctcattacct tgcagtattt ttctctggaa
181 atccttgtaa tcttgaagga atggacctca aaattatggc atcgtcaaag cattgtggtg
241 tcttttttac tgctgcttgc tgtgcttata gctacgtatt atgttgaagg agtgcatcaa
301 cagtatgtgc aacgtataga gaaacagttt cttttgtatg cctactggat aggcttagga
361 attttgtctt ctgttgggct tggaacaggg ctgcacacct ttctgcttta tctgggtcca
421 catatagcct cagttacatt agctgcttat gaatgcaatt cagttaattt tcccgaacca
481 ccctatcctg atcagattat ttgtccagat gaagagggca ctgaaggaac catttttttg
541 tggagtatca tctcaaaagt taggattgaa gcctgcatgt ggggtatcgg tacagcaatc
601 ggagagctgc ctccatattt catggccaga gcagctcgcc tctcaggtgc tgaaccagat
661 gatgaagagt atcaggaatt tgaagagatg ctggaacatg cagagtctgc acaagacttt
721 gcctcccggg ccaaactggc agttcaaaaa ctagtacaga aagttggatt ttttggaatt
781 ttggcctgtg cttcaattcc aaatccttta tttgatctgg ctggaataac gtgtggacac
841 tttctggtac ctttttggac cttctttggt gcaaccctaa ttggaaaagc aataataaaa
901 atgcatatcc agaaaatttt tgttataata acattcagca agcacatagt ggagcaaatg
961 gtggctttca ttggtgctgt ccccggcata ggtccatctc tgcagaagcc atttcaggag
1021 tacctggagg ctcaacggca gaagcttcac cacaaaagcg aaatgggcac accacaggga
1081 gaaaactggt tgtcctggat gtttgaaaag ttggtcgttg tcatggtgtg ttacttcatc
1141 ctatctatca ttaactccat ggcacaaagt tatgccaaac gaatccagca gcggttgaac
1201 tcagaggaga aaactaaata agtagagaaa gttttaaact gcagaaattg gagtggatgg
1261 gttctgcctt aaattgggag gactccaagc cgggaaggaa a
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