LOCUS CR533519 1198 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1119D for
gene GHITM, growth hormone inducible transmembrane protein;
complete cds, incl. stopcodon.
ACCESSION CR533519
VERSION CR533519.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1038)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1038)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E1119D, ORFNo 2865
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1119D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136713
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1198
/db_xref="H-InvDB:HIT000268367"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E1119D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1038
/codon_start=1
/gene="GHITM"
/db_xref="GOA:Q6FIA7"
/db_xref="H-InvDB:HIT000268367.12"
/db_xref="InterPro:IPR006214"
/db_xref="InterPro:IPR035871"
/db_xref="UniProtKB/TrEMBL:Q6FIA7"
/protein_id="CAG38550.1"
/translation="MLAVRLVCLRTLPSRVFHPAFTKASPVVKNSITKNQWLLTPSRE
YATKTRIGIRRGRTGQELKEAALEPSMEKMFKIDQMGRWFVAGGAAVGLGALCYYGLG
LSNEIGAIEKAVIWPRYVKDRIHSTYMYLAGSIGLTALSAIAISRTPVLMNFMMRGSW
VTIGVTFAAMVGAGMLVRSIPYDQSPGPKHLAWLLHSGVMGAVVAPLTILGGPLLIRA
AWYTAGIVGGLSTVAMCAPSEKFLNMGAPLGVGLGLVFVSSLGSMFLPPTTVAGATLY
SVAMYGGLVLFSMFLLYDTQKVIKRAEVSPMYGVQKYDPINSMLSIYMDTLNIFMRVA
TMLATGGNRKK"
BASE COUNT 303 a 246 c 306 g 343 t
ORIGIN
1 atgttggctg taaggctggt gtgtctccgg acactacctt ctagggtttt ccacccagct
61 ttcaccaagg cctcccctgt tgtgaagaat tccatcacga agaatcaatg gctgttaaca
121 cctagcaggg aatatgccac caaaacaaga attgggatcc ggcgtgggag aactggccaa
181 gaactcaaag aggcagcatt ggaaccatcg atggaaaaaa tgtttaaaat tgatcagatg
241 ggaagatggt ttgttgctgg aggggctgct gttggtcttg gagcattgtg ctactatggc
301 ttgggactgt ctaatgagat tggagctatt gaaaaggctg taatttggcc tcggtatgtc
361 aaggatagaa ttcattccac ctatatgtac ttagcaggga gtattggttt aacagctttg
421 tctgccatag caatcagcag aacgcctgtt ctcatgaact tcatgatgag aggctcttgg
481 gtgacaattg gtgtgacctt tgcagccatg gttggagctg gaatgctggt acgatcaata
541 ccatatgacc agagcccagg cccaaagcac cttgcttggt tgctacattc tggtgtgatg
601 ggtgcagtgg tggctcctct gacaatatta gggggtcctc ttctcatcag agctgcatgg
661 tacacagctg gcattgtggg aggcctctcc actgtggcca tgtgtgcgcc cagtgaaaag
721 tttctgaaca tgggtgcacc cctgggagtg ggcctgggtc tcgtctttgt gtcctcattg
781 ggatctatgt ttcttccacc taccaccgtg gctggtgcca ctctttactc agtggcaatg
841 tacggtggat tagttctttt cagcatgttc cttctgtatg atacccagaa agtaatcaag
901 cgtgcagaag tatcaccaat gtatggagtt caaaaatatg atcccattaa ctcgatgctg
961 agtatctaca tggatacatt aaatatattt atgcgagttg caactatgct ggcaactgga
1021 ggcaacagaa agaaatgaag tgactcagct tctggcttct ctgctacatc aaatatcttg
1081 tttaatgggg cagatatgca ttaaatagtt tgtacaagca gctttcgttg aagtttagaa
1141 gataagaaac atgtcatcat atttaaatgt tccggtaatg tgatgcctca ggtctgcc
//