LOCUS CR533516 906 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1119D for
gene OGN, osteoglycin (osteoinductive factor, mimecan); complete
cds, incl. stopcodon.
ACCESSION CR533516
VERSION CR533516.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 897)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 897)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1119D, ORFNo 2860
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1119D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence NM_014057 (gi14916496)
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..906
/db_xref="H-InvDB:HIT000268364"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1119D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..897
/codon_start=1
/gene="OGN"
/db_xref="GOA:P20774"
/db_xref="H-InvDB:HIT000268364.12"
/db_xref="HGNC:HGNC:8126"
/db_xref="InterPro:IPR001611"
/db_xref="InterPro:IPR003591"
/db_xref="InterPro:IPR027211"
/db_xref="InterPro:IPR032675"
/db_xref="UniProtKB/Swiss-Prot:P20774"
/protein_id="CAG38547.1"
/translation="MKTLQSTLLLLLLVPLIKPAPPTQQDSRIIYDYGTDNFEESIFS
QDYEDKYLDGKNIKEKETVIIPNEKSLQLQKDEAITPLPPKKENDEMPTCLLCVCLSG
SVYCEEVDIDAVPPLPKESAYLYARFNKIKKLTAKDFADIPNLRRLDFTGNLIEDIED
GTFSKLSLLEELSLAENQLLKLPVLPPKLTLFNAKYNKIKSRGIKANAFKKLNNLTFL
YLDHNALESVPLNLPESLRVIHLQFNNIASITDDTFCKANDTSYIRDRIEEIRLEGNP
IVLGKHPNSFICLKRLPIGSYF"
BASE COUNT 317 a 192 c 152 g 245 t
ORIGIN
1 atgaagactc tgcagtctac acttctcctg ttactgcttg tgcctctgat aaagccagca
61 ccaccaaccc agcaggactc acgcattatc tatgattatg gaacagataa ttttgaagaa
121 tccatattta gccaagatta tgaggataaa tacctggatg gaaaaaatat taaggaaaaa
181 gaaactgtga taatacccaa tgagaaaagt cttcaattac aaaaagatga ggcaataaca
241 ccattacctc ccaagaaaga aaatgatgaa atgcccacgt gtctgctgtg tgtttgttta
301 agtggctctg tatactgtga agaagttgac attgatgctg taccaccctt accaaaggaa
361 tcagcctatc tttacgcacg attcaacaaa attaaaaagc tgactgccaa agattttgca
421 gacataccta acttaagaag actcgatttt acaggaaatt tgatagaaga tatagaagat
481 ggtacttttt caaaactttc tctgttagaa gaactttcac ttgctgaaaa tcaactacta
541 aaacttccag ttcttcctcc caagctcact ttatttaatg caaaatacaa caaaatcaag
601 agtaggggaa tcaaagcaaa tgcattcaaa aaactgaata acctcacctt cctctacttg
661 gaccataatg ccctggaatc cgtgcctctt aatttaccag aaagtctacg tgtaattcat
721 cttcagttca acaacatagc ttcaattaca gatgacacat tctgcaaggc taatgacacc
781 agttacatcc gggaccgcat tgaagagata cgcctggagg gcaatccaat cgtcctggga
841 aagcatccaa acagttttat ttgcttaaaa agattaccga tagggtcata cttttaacct
901 ctattg
//