LOCUS CR533513 1156 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A1019D for
gene RNF32, ring finger protein 32; complete cds, incl. stopcodon.
ACCESSION CR533513
VERSION CR533513.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1089)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1089)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A1019D, ORFNo 2852
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A1019D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence NM_030936 (gi37595535)
we found AA exchange(s) at position (first base of changed
triplet):
289(leu->pro)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1156
/db_xref="H-InvDB:HIT000268361"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A1019D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1089
/codon_start=1
/gene="RNF32"
/db_xref="GOA:Q9H0A6"
/db_xref="H-InvDB:HIT000268361.14"
/db_xref="HGNC:HGNC:17118"
/db_xref="InterPro:IPR000048"
/db_xref="InterPro:IPR001841"
/db_xref="InterPro:IPR013083"
/db_xref="InterPro:IPR027370"
/db_xref="UniProtKB/Swiss-Prot:Q9H0A6"
/protein_id="CAG38544.1"
/translation="MLKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQV
QKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKEYVLDPKPPPLTLAQKLGPIGPPP
PPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFT
NKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDA
KLRKKFFEKKFTEISHRILCSYNTNIEELFAEIDQCLAINRSVLQQLEEKCGHEITEE
EWEKIQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLAL
EEFSVGDRPPFHACPLCRSCYQKKILEC"
BASE COUNT 355 a 277 c 257 g 267 t
ORIGIN
1 atgttaaaaa ataagggtca ctcatctaag aaagataact tggcagtcaa tgcagttgct
61 ttacaagatc acattttaca tgatcttcaa cttcgaaatc tttcagttgc agatcattct
121 aagacacaag tacaaaagaa agagaacaaa tctctaaaaa gagatacaaa ggcaataata
181 gatactggac ttaaaaaaac tacacagtgc cccaaactag aagactcaga aaaagaatat
241 gttcttgatc ccaaaccgcc gccgttgact ttggcacaga agttgggccc cattgggcct
301 ccaccacctc cactgtcatc agatgaatgg gagaaggtga aacagcgctc tctcctgcaa
361 ggggactccg tgcaaccatg ccccatctgt aaagaagaat tcgagcttcg tcctcaggtg
421 ctgctttcat gctcccatgt gttccacaaa gcatgtcttc aggcttttga aaagttcaca
481 aataagaaaa cctgtcctct ctgtagaaag aaccagtatc aaacccgagt gatacacgat
541 ggggcccgcc tgttcagaat caagtgtgtg accagaatcc aagcctactg gagaggatgt
601 gttgttagaa agtggtacag aaacctgagg aaaacagtac ctcccacaga tgccaagtta
661 agaaaaaaat tctttgaaaa aaagttcaca gaaatcagcc accgcatcct gtgctcatac
721 aacaccaaca ttgaagagct ctttgcagaa atcgatcagt gcttggccat aaatcgaagt
781 gttcttcagc agttggaaga aaaatgtggc catgagatca cagaagagga atgggagaaa
841 atccaagtgc aggctctgcg ccgggagacc cacgagtgct ccatctgcct ggcccctctc
901 tccgctgctg gcggtcagcg cgtgggtgca ggcaggcgtt ccagagagat ggccctcctg
961 tcctgctcac atgtgttcca ccatgcgtgc ctgctggcac tagaggagtt ctccgtggga
1021 gacaggcctc ctttccatgc ctgtcctctc tgccgctcct gctaccagaa gaagattctt
1081 gaatgttgaa ttcatagtca aggaaagtta ggtaattctg agaaaaaaag tttaccatca
1141 ttttggatga actgca
//