LOCUS CR533508 1485 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0819D for
gene MGC8407, hypothetical protein MGC8407; complete cds, incl.
stopcodon.
ACCESSION CR533508
VERSION CR533508.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1422)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1422)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0819D, ORFNo 2846
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0819D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136697
we found AA exchange(s) at position (first base of changed
triplet):
64(asp->gly)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1485
/db_xref="H-InvDB:HIT000268356"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0819D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1422
/codon_start=1
/gene="MGC8407"
/db_xref="GOA:Q8NCB2"
/db_xref="H-InvDB:HIT000268356.13"
/db_xref="HGNC:HGNC:28788"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR011009"
/db_xref="UniProtKB/Swiss-Prot:Q8NCB2"
/protein_id="CAG38539.1"
/translation="MPFGCVTLGDKKNYNQPSEVTGRYDLGQVIKTEEFCEIFRAKDK
TTGKLHTCKKFQKRDGRKVRKAAKNEIGILKMVKHPNILQLVDVFVTRKEYFIFLELA
TGREVFDWILDQGYYSERDTSNVVRQVLEAVAYLHSLKIVHRNLKLENLVYYNRLKNS
KIVISDFHLAKLENGLIKEPCGTPEYLGNPPFYEEVEEDDYENHDKNLFRKILAGDYE
FDSPYWDDISQAAKDLVTRLMEVEQDQRITAEEAISHEWISGNAASDKNIKDGVCAQI
EKNFARAKWKKAVRVTTLMKRLRAPEQSSTAAAQSASATDTATPGAAGGATAAAASGA
TSAPEGDAARAAKSDNVAPADRSATPATDGSATPATDGSVTPATDGSITPATDGSVTP
ATDRSATPATDGRATPATEESTVPTTQSSAMLATKAAATPEPAMAQPDSTAPEGATGQ
APPSSKGEEAAGYAQESQREEAS"
BASE COUNT 374 a 396 c 455 g 260 t
ORIGIN
1 atgccgtttg ggtgtgtgac tctgggtgac aagaagaact ataaccagcc atcggaggtg
61 actggcagat atgatttggg acaggtcatc aagactgagg agttttgtga aatcttccgg
121 gccaaggaca agacgacagg caagctgcac acctgcaaga agttccagaa gcgggacggc
181 cgcaaggtgc ggaaagctgc caagaacgag ataggcatcc tcaagatggt gaagcatccc
241 aacatcctac agctggtgga tgtgtttgtg acccgcaagg agtactttat cttcctggag
301 ctggccacgg ggagggaggt gtttgactgg atcctggacc agggctacta ctcggagcga
361 gacacaagca acgtggtacg gcaagtcctg gaggccgtgg cctatttgca ctcactcaag
421 atcgtgcaca ggaatctcaa gctggagaac ctggtttact acaaccggct gaagaactcg
481 aagattgtca tcagtgactt ccatctggct aagctagaaa atggcctcat caaggagccc
541 tgtgggaccc ccgagtatct gggcaaccca cctttctatg aggaggtgga agaagatgat
601 tatgagaacc atgataagaa tctcttccgc aagatcctgg ctggtgacta tgagtttgac
661 tctccatatt gggatgatat ttcgcaggca gccaaagacc tggtcacaag gctgatggag
721 gtggagcaag accagcggat cactgcagaa gaggccatct cccatgagtg gatttctggc
781 aatgctgctt ctgataagaa catcaaggat ggtgtctgtg cccagattga aaagaacttt
841 gccagggcca agtggaagaa ggctgtccga gtgaccaccc tcatgaaacg gctccgggca
901 ccagagcagt ccagcacggc tgcagcccag tcggcctcag ccacagacac tgccaccccc
961 ggggctgcag gtggggccac agctgcagct gcgagtggag ctacctcagc ccctgagggt
1021 gatgctgctc gtgctgcaaa gagtgataat gtggcccccg cagaccgtag tgccacccca
1081 gccacagatg gaagtgccac cccagccact gatggcagtg tcaccccagc caccgatgga
1141 agcatcactc cagccactga tgggagtgtc accccagcca ctgacaggag cgctactcca
1201 gccactgatg ggagagccac accagccaca gaagagagca ctgtgcccac cacccaaagc
1261 agtgccatgc tggccaccaa ggcagctgcc acccctgagc cggctatggc ccagccggac
1321 agcacagccc cagagggcgc cacaggccag gctccaccct ctagtaaagg ggaagaggct
1381 gctggttatg cccaggagtc tcaaagggag gaggccagct gagtaggcag cctggtgagg
1441 gggggcaggg gatgggcagg agggtgggag agtggatgag gggct
//