LOCUS CR533492 719 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B1217D for
gene SARA1, SAR1a gene homolog 1 (S. cerevisiae); complete cds,
incl. stopcodon.
ACCESSION CR533492
VERSION CR533492.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 597)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 597)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B1217D, ORFNo 2819
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B1217D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence NM_020150 (gi21361614)
we found AA exchange(s) at position (first base of changed
triplet):
310(asp->gly) 484(asn->asp) 550(gln->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..719
/db_xref="H-InvDB:HIT000268340"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B1217D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..597
/codon_start=1
/gene="SARA1"
/db_xref="GOA:Q6FID4"
/db_xref="H-InvDB:HIT000268340.13"
/db_xref="InterPro:IPR005225"
/db_xref="InterPro:IPR006687"
/db_xref="InterPro:IPR006689"
/db_xref="InterPro:IPR027417"
/db_xref="UniProtKB/TrEMBL:Q6FID4"
/protein_id="CAG38523.1"
/translation="MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHM
LKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDC
AGHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGK
GDVTLKELNARPMEVFMCSVLKRRGYGEGFRWLSQYID"
BASE COUNT 201 a 150 c 169 g 199 t
ORIGIN
1 atgtctttca tctttgagtg gatctacaat ggcttcagca gtgtgctcca gttcctagga
61 ctgtacaaga aatctggaaa acttgtattc ttaggtttgg ataatgcagg caaaaccact
121 cttcttcaca tgctcaaaga tgacagattg ggccaacatg ttccaacact acatccgaca
181 tcagaagagc taacaattgc tggaatgacc tttacaactt ttgatcttgg tgggcacgag
241 caagcacgtc gcgtttggaa aaattatctc ccagcaatta atgggattgt ctttctggtg
301 gactgtgcag gtcattctcg cctcgtggaa tccaaagttg agcttaatgc tttaatgact
361 gatgagacaa tatccaatgt gccaatcctt atcttgggta acaaaattga cagaacagat
421 gcaatcagtg aagaaaaact ccgtgagata tttgggcttt atggacagac cacaggaaag
481 ggggatgtga ccctgaagga gctgaatgct cgccccatgg aagtgttcat gtgcagtgtg
541 ctcaagaggc gaggttacgg cgagggtttc cgctggctct cccagtatat tgactgatgt
601 ttggacggtg aaaataaaag agttttactt ctctggactg atcctattca cagcttcctc
661 atgaactttt ctaatagaac aaggatagct ctccaaccat gtctggcgtt gagaagcca
//