LOCUS CR533491 631 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1117D for
gene MRPL18, mitochondrial ribosomal protein L18; complete cds,
incl. stopcodon.
ACCESSION CR533491
VERSION CR533491.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 543)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 543)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1117D, ORFNo 2817
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1117D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AL136633
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..631
/db_xref="H-InvDB:HIT000268339"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1117D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..543
/codon_start=1
/gene="MRPL18"
/db_xref="GOA:Q9H0U6"
/db_xref="H-InvDB:HIT000268339.12"
/db_xref="HGNC:HGNC:14477"
/db_xref="InterPro:IPR005484"
/db_xref="InterPro:IPR036967"
/db_xref="PDB:3J7Y"
/db_xref="PDB:5OOL"
/db_xref="PDB:5OOM"
/db_xref="PDB:6NU2"
/db_xref="PDB:6NU3"
/db_xref="UniProtKB/Swiss-Prot:Q9H0U6"
/protein_id="CAG38522.1"
/translation="MALRSRFWGLFSVCRNPGCRFAALSTSSEPAAKPEVDPVENEAV
APEFTNRNPRNLELLSVARKERGWRTVFPSREFWHRLRVIRTQHHVEALVEHQNGKVV
VSASTREWAIKKHLYSTRNVVACESIGRVLAQRCLEAGINFMVYQPTPWEAASDSMKR
LQSAMTEGGVVLREPQRIYE"
BASE COUNT 165 a 144 c 183 g 139 t
ORIGIN
1 atggcgcttc ggtcgcggtt ttgggggttg ttctcggttt gcaggaaccc tgggtgcagg
61 ttcgcagccc tgtcaaccag ctccgagccg gcagcgaaac ctgaagtgga ccctgtggaa
121 aatgaagctg tcgccccaga attcaccaac cggaaccccc ggaacctgga gcttttgtct
181 gtagccagga aagagcgggg ctggcggacg gtgtttccct cccgtgagtt ctggcacagg
241 ttgcgagtta taaggactca gcatcatgta gaagcacttg tggagcatca gaatggcaag
301 gttgtggttt cggcctccac tcgtgagtgg gctattaaaa agcaccttta tagtaccaga
361 aatgtggtgg cttgtgagag tataggacga gtgctggcac agagatgctt agaggcggga
421 atcaacttca tggtctacca accaaccccg tgggaggcag cctcagactc gatgaaacga
481 ctacaaagtg ccatgacaga aggtggtgtg gttctacggg aacctcagag aatctatgaa
541 taaatggaag cattaattgt tttgaacatg taaatataaa tctgtcagcc actacagcca
601 tcaaaagaga gcatctggaa gaacagccag c
//