LOCUS CR533464 823 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0317D for
gene UNC50, unc-50 homolog (C. elegans); complete cds, incl.
stopcodon.
ACCESSION CR533464
VERSION CR533464.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 780)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 780)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (21-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0317D, ORFNo 2775
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0317D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full ORF clones
generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
After the stop codon 3' UTR sequence is present in front of the
3' att site (ACCCAGCTTTCTT).
Compared to the reference sequence AY017215
we did not find any amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..823
/db_xref="H-InvDB:HIT000268312"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0317D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..780
/codon_start=1
/gene="UNC50"
/db_xref="GOA:Q53HI1"
/db_xref="H-InvDB:HIT000268312.11"
/db_xref="HGNC:HGNC:16046"
/db_xref="InterPro:IPR007881"
/db_xref="UniProtKB/Swiss-Prot:Q53HI1"
/protein_id="CAG38495.1"
/translation="MLPSTSVNSLVQGNGVLNSRDAARHTAGAKRYKYLRRLFRFRQM
DFEFAAWQMLYLFTSPQRVYRNFHYRKQTKDQWARDDPAFLVLLSIWLCVSTIGFGFV
LDMGFFETIKLLLWVVLIDCVGVGLLIATLMWFISNKYLVKRQSRDYDVEWGYAFDVH
LNAFYPLLVILHLIQLFFINHVILTDTFIGYLVGNTLWLVAVGYYIYVTFLGYSALPF
LKNTVILLYPFAPLILLYGLSLALGWNFTHTLCSFYKYRVK"
BASE COUNT 208 a 164 c 181 g 270 t
ORIGIN
1 atgttaccga gtacttcagt gaattcctta gtgcagggga acggagtctt gaattccagg
61 gatgcggcaa gacacacagc cggagcgaaa cgctacaaat atctgagaag gcttttccgc
121 tttcggcaaa tggactttga atttgctgcc tggcagatgc tctacctgtt cacatcccca
181 cagagagttt acagaaattt tcattatcga aaacagacga aggaccagtg ggccagagat
241 gaccctgctt tcttggtcct gttaagtatc tggctctgtg tgtccactat aggatttggc
301 tttgtgctgg acatgggatt ctttgagaca ataaagcttc tcctttgggt tgtactcata
361 gattgtgtag gcgttggtct tctgatagca actttaatgt ggttcatctc taacaagtat
421 ttagtgaaac gacagagcag agactatgat gtggaatggg gctatgcttt tgatgtgcat
481 ctcaatgctt tttatccact cctggtcatt ttgcatctta tccagctttt tttcatcaac
541 catgttatcc tgacagacac atttattgga tatttagttg gaaatacctt atggttggtt
601 gcagttggct attatatcta tgtaactttc ctgggataca gtgcattgcc atttttgaaa
661 aatacagtaa ttcttctgta tccatttgca cctctgattc tgctctacgg gctttccctg
721 gcactgggat ggaacttcac ccatactctc tgttctttct ataagtacag agtgaaataa
781 aaagtgagaa gaagattcaa tcgtaactgt gtctacagta ttg
//