LOCUS CR457437 840 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D076D for
gene FHL2, four and a half LIM domains 2; complete cds, incl.
stopcodon.
ACCESSION CR457437
VERSION CR457437.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 840)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 840)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D076D, ORFNo 2727
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D076D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BT006960 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..840
/db_xref="H-InvDB:HIT000268287"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D076D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..840
/codon_start=1
/gene="FHL2"
/db_xref="GOA:Q6I9R8"
/db_xref="H-InvDB:HIT000268287.13"
/db_xref="InterPro:IPR001781"
/db_xref="InterPro:IPR037987"
/db_xref="UniProtKB/TrEMBL:Q6I9R8"
/protein_id="CAG33718.1"
/translation="MTERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECG
KPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQ
ECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAM
QCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYA
KKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCG
KDI"
BASE COUNT 203 a 232 c 227 g 178 t
ORIGIN
1 atgactgagc gctttgactg ccaccattgc aacgaatctc tctttggcaa gaagtacatc
61 ctgcgggagg agagccccta ctgcgtggtg tgctttgaga ccctgttcgc caacacctgc
121 gaggagtgtg ggaagcccat cggctgtgac tgcaaggact tgtcttacaa ggaccggcac
181 tggcatgaag cctgtttcca ctgctcgcag tgcagaaact cactggtgga caagcccttt
241 gctgccaagg aggaccagct gctctgtaca gactgctatt ccaacgagta ctcatccaag
301 tgccaggaat gcaagaagac catcatgcca ggtacccgca agatggagta caagggcagc
361 agctggcatg agacctgctt catctgccac cgctgccagc agccaattgg aaccaagagt
421 ttcatcccca aagacaatca gaatttctgt gtgccctgct atgagaaaca acatgccatg
481 cagtgcgttc agtgcaaaaa gcccatcacc acgggagggg tcacttaccg ggagcagccc
541 tggcacaagg agtgcttcgt gtgcaccgcc tgcaggaagc agctgtctgg gcagcgcttc
601 acagctcgcg atgactttgc ctactgcctg aactgcttct gtgacttgta tgccaagaag
661 tgtgctgggt gcaccaaccc catcagcgga cttggtggca caaaatacat ctcctttgag
721 gaacggcagt ggcataacga ctgctttaac tgtaagaagt gctccctctc actggtgggg
781 cgtggcttcc tcacagagag ggatgacatc ctgtgccccg actgtgggaa agacatttaa
//