LOCUS CR457432 1074 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0619D for
gene NOV, nephroblastoma overexpressed gene; complete cds, incl.
stopcodon.
ACCESSION CR457432
VERSION CR457432.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1074)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1074)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0619D, ORFNo 2705
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0619D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC015028 we found amino acid
exchange(s) at position (first base of changed triplet):
1069(met->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1074
/db_xref="H-InvDB:HIT000268282"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0619D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1074
/codon_start=1
/gene="NOV"
/db_xref="GOA:P48745"
/db_xref="H-InvDB:HIT000268282.12"
/db_xref="HGNC:HGNC:7885"
/db_xref="InterPro:IPR000867"
/db_xref="InterPro:IPR000884"
/db_xref="InterPro:IPR001007"
/db_xref="InterPro:IPR006207"
/db_xref="InterPro:IPR006208"
/db_xref="InterPro:IPR009030"
/db_xref="InterPro:IPR012395"
/db_xref="InterPro:IPR017891"
/db_xref="InterPro:IPR036383"
/db_xref="UniProtKB/Swiss-Prot:P48745"
/protein_id="CAG33713.1"
/translation="MQSVQSTSFCLRKQCLCLTFLLLHLLGQVAATQRCPPQCPGRCP
ATPPTCAPGVRAVLDGCSCCLVCARQRGESCSDLEPCDESSGLYCDRSADPSKQTGIC
TAVEGDNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGCVPRCQLDVLLPEPNCPAPRK
VEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGVEVSDSSVNCIEQTTEWTACSK
SCGMGFSTRVTNRNRQCEMLKQTRLCMVRPCEQEPEQPTDKKGKKCLRTKKSLKAIHL
QFKNCTSLHTYKPRFCGVCSDGRCCTPHNTKTIQAEFQCSPGQIVKKPVMVIGTCTCH
TNCPKNNEAFLQELELKTTRGKI"
BASE COUNT 254 a 305 c 309 g 206 t
ORIGIN
1 atgcagagtg tgcagagcac gagcttttgt ctccgaaagc agtgcctttg cctgaccttc
61 ctgcttctcc atctcctggg acaggtcgct gcgactcagc gctgccctcc ccagtgcccg
121 ggccggtgcc ctgcgacgcc gccgacctgc gcccccgggg tgcgcgcggt gctggacggc
181 tgctcatgct gtctggtgtg tgcccgccag cgtggcgaga gctgctcaga tctggagcca
241 tgcgacgaga gcagtggcct ctactgtgat cgcagcgcgg accccagcaa acagactggc
301 atctgcacgg cggtagaggg agataactgt gtgttcgatg gggtcatcta ccgcagtgga
361 gagaaatttc agccaagctg caaattccag tgcacctgca gagatgggca gattggctgt
421 gtgccccgct gtcagctgga tgtgctactg cctgagccta actgcccagc tccaagaaaa
481 gttgaggtgc ctggagagtg ctgtgaaaag tggatctgtg gcccagatga ggaggattca
541 ctgggaggcc ttacccttgc agcttacagg ccagaagcca ccctaggagt agaagtctct
601 gactcaagtg tcaactgcat tgaacagacc acagagtgga cagcatgctc caagagctgt
661 ggtatggggt tctccacccg ggtcaccaat aggaaccgtc aatgtgagat gctgaaacag
721 actcggctct gcatggtgcg gccctgtgaa caagagccag agcagccaac agataagaaa
781 ggaaaaaagt gtctccgcac caagaagtca ctcaaagcca tccacctgca gttcaagaac
841 tgcaccagcc tgcacaccta caagcccagg ttctgtgggg tctgcagtga tggccgctgc
901 tgcactcccc acaataccaa aaccatccag gcagagtttc agtgctcccc agggcaaata
961 gtcaagaagc cagtgatggt cattgggacc tgcacctgtc acaccaactg tcctaagaac
1021 aatgaggcct tcctccagga gctggagctg aagactacca gagggaaaat ttaa
//