LOCUS CR457398 930 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0514D for
gene DKFZP566F0546, DKFZP566F0546 protein; complete cds, incl.
stopcodon.
ACCESSION CR457398
VERSION CR457398.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 930)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 930)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0514D, ORFNo 2576
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0514D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_015653 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..930
/db_xref="H-InvDB:HIT000268248"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0514D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..930
/codon_start=1
/gene="DKFZP566F0546"
/db_xref="GOA:Q9H4K1"
/db_xref="H-InvDB:HIT000268248.12"
/db_xref="HGNC:HGNC:13241"
/db_xref="InterPro:IPR008805"
/db_xref="UniProtKB/Swiss-Prot:Q9H4K1"
/protein_id="CAG33679.1"
/translation="MRQNDKIMCILENRKKRDRKNLCRAINDFQQSFQKPETRREFDL
SDPLALKKDLPARQSDNDVRNTISGMQKFMGEDLNFHERKKFQEEQNREWSLQQQREW
KNARAEQKCAEALYTETRLQFDETAKHLQKLESTTRKAVCASVKDFNKSQAIESVERK
KQEKKQEQEDNLAEITNLLRGDLLSENPQQAASSFGPHRVVPDRWKGMTQEQLEQIRL
VQKQQIQEKLRLQEEKRQRDLDWDRRRIQGARATLLFERQQWRRQRDLRRALDSSNLS
LAKEQHLQKKYMNEVYTNQPTGDYFTQFNTGSR"
BASE COUNT 301 a 228 c 254 g 147 t
ORIGIN
1 atgaggcaaa atgacaaaat catgtgcata ttggaaaacc ggaaaaagag ggataggaaa
61 aatctctgta gggctatcaa tgacttccaa cagagctttc agaagccaga aactcgccgt
121 gaatttgatc tgtccgaccc cctagccctt aagaaagatc ttccagcccg gcagtcagat
181 aatgatgttc ggaatacgat atcaggaatg cagaaattca tgggagagga tttaaacttc
241 catgagagga agaaattcca agaggaacaa aacagagaat ggtctttgca gcagcaaagg
301 gaatggaaga acgcccgtgc tgaacaaaaa tgcgcagagg ccctctacac agagacaagg
361 ctgcagtttg acgagacagc caagcacctc cagaagctgg aaagcaccac cagaaaggca
421 gtttgtgcat ctgtgaaaga cttcaacaag agccaggcca tcgagtcagt ggaaaggaaa
481 aagcaagaga aaaagcaaga acaagaggac aacttggccg agatcaccaa cctcctgcgt
541 ggggacctgc tctccgagaa cccgcagcag gcagccagct ccttcgggcc ccaccgcgtg
601 gtccctgacc gctggaaggg catgacccag gagcagctgg agcagatccg cctagtccag
661 aagcagcaaa tccaggagaa gctgaggctc caggaagaaa agcgccagcg agacctggac
721 tgggaccggc ggaggattca gggggctcgc gccaccctgc tgtttgagcg gcagcagtgg
781 cggcggcagc gcgacctgcg cagagctctg gacagcagca acctcagcct ggccaaggag
841 cagcatttgc agaaaaaata tatgaatgaa gtctatacaa atcaacccac gggagactat
901 ttcacacaat ttaatacagg aagtcgttaa
//