LOCUS CR457386 426 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0510D for
gene DKFZP586N0721, DKFZP586N0721 protein; complete cds, incl.
stopcodon.
ACCESSION CR457386
VERSION CR457386.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 426)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 426)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F0510D, ORFNo 2549
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0510D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_015400 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..426
/db_xref="H-InvDB:HIT000268236"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F0510D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..426
/codon_start=1
/gene="DKFZP586N0721"
/db_xref="H-InvDB:HIT000268236.13"
/db_xref="UniProtKB/TrEMBL:Q6I9W9"
/protein_id="CAG33667.1"
/translation="MACTYVSNLGKKQRSVSFLASGLMRVSTGPELRLHHSFVLTGDV
GRRICRLLVGLFTKGDTSSKRVHPFSPGPCFLLCDLARVGSSPKINVSPFYQNQTSTQ
RSCTVFVWQRCSLVGPFQVTVFTMYFHHSLRSISRFSSG"
BASE COUNT 106 a 105 c 104 g 111 t
ORIGIN
1 atggcatgca cgtatgtaag taatctgggg aagaagcaaa gatctgtttc attcttagcc
61 tcaggcctca tgagggtctc cacagggccg gagctcaggt tacaccactc cttcgtcctt
121 acaggagatg tagggagaag aatctgcagg ctgcttgtag gactgttcac caagggggat
181 accagcagca agagagtgca cccgtttagc cctggaccct gtttcttact gtgtgacttg
241 gctagagttg ggagttcccc caaaataaac gtgtccccat tttaccagaa ccaaacctca
301 acacagcgaa gctgtactgt ctttgtgtgg caaagatgtt cccttgtagg cccctttcag
361 gtaaccgtct tcacaatgta ttttcatcac agtttaagga gcatcagccg cttctcaagt
421 ggttaa
//