LOCUS CR457381 1428 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0314D for
gene E46L, like mouse brain protein E46; complete cds, incl.
stopcodon.
ACCESSION CR457381
VERSION CR457381.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1428)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1428)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0314D, ORFNo 2536
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0314D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_013236 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1428
/db_xref="H-InvDB:HIT000268231"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0314D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1428
/codon_start=1
/gene="E46L"
/db_xref="GOA:Q9UBB4"
/db_xref="H-InvDB:HIT000268231.13"
/db_xref="HGNC:HGNC:10549"
/db_xref="InterPro:IPR011989"
/db_xref="InterPro:IPR016024"
/db_xref="InterPro:IPR019156"
/db_xref="UniProtKB/Swiss-Prot:Q9UBB4"
/protein_id="CAG33662.1"
/translation="MAAPRPPPARLSGVMVPAPIQDLEALRALTALFKEQRNRETAPR
TIFQRVLDILKKSSHAVELACRDPSQVENLASSLQLITECFRCLRNACIECSVNQNSI
RNLDTIGVAVDLILLFRELRVEQESLLTAFRCGLQFLGNIASRNEDSQSIVWVHAFPE
LFLSCLNHPDKKIVAYSSMILFTSLNHERMKELEENLNIAIDVIDAYQKHPESEWPFL
IITDLFLKSPELVQAMFPKLNNQERVTLLDLMIAKITSDEPLTKDDIPVFLRHAELIA
STFVDQCKTVLKLASEEPPDDEEALATIRLLDVLCEMTVNTELLGYLQVFPGLLERVI
DLLRVIHVAGKETTNIFSNCGCVRAEGDISNVANGFKSHLIRLIGNLCYKNKDNQDKV
NELDGIPLILDNCNISDSNPFLTQWVIYAIRNLTEDNSQNQDLIAKMEEQGLADASLL
KKVGFEVEKKGEKLILKSTRDTPKP"
BASE COUNT 393 a 318 c 342 g 375 t
ORIGIN
1 atggcggcgc ccaggccgcc gcctgccagg ctgtcgggcg tcatggtgcc ggcgcccatc
61 caagacctgg aggccctgcg cgcgctcacg gcgctcttca aagagcagcg gaaccgagaa
121 acagcaccca ggactatctt ccaaagagtt ctggatatcc taaagaaatc ttctcatgct
181 gttgagcttg cctgcagaga tccatcccaa gtggaaaacc tggcttccag tctgcagtta
241 ataacagaat gcttcaggtg tcttcgcaat gcttgcatag agtgttctgt gaaccagaat
301 tcaatcagga acttggatac gattggtgtt gctgttgatt tgattcttct gtttcgtgaa
361 ctgcgagtgg aacaggaatc tctgttgaca gcttttcgct gtggcctgca gtttttaggc
421 aacattgcct cacggaatga agattcccag tctattgttt gggtgcatgc tttcccagaa
481 ctgtttttgt cttgcttaaa tcatccggac aaaaaaattg ttgcctactc ttcaatgatt
541 ttgtttacat cccttaatca tgaaagaatg aaagaactgg aggagaacct caatattgca
601 attgatgtca tagatgctta ccaaaaacat cctgaatcag aatggccgtt cttgattatt
661 acagacctct ttctgaaaag cccggaattg gtacaagcca tgtttcccaa actgaacaat
721 caagaaagag ttacactgtt agaccttatg atagccaaga taacgagtga tgagccactc
781 accaaggatg acatccctgt gtttttgcgg catgctgagt tgattgcaag cacctttgtg
841 gatcagtgca agactgtgct caagctggcc tctgaggagc ctcctgatga tgaggaggca
901 ctggctacaa ttaggcttct cgacgtcctg tgcgaaatga ctgtgaatac tgagctgctc
961 ggctatctgc aggttttccc tggcttgctg gaaagagtga ttgatctttt gcgggtgatt
1021 catgtagctg gaaaagaaac cacaaacatc ttcagtaatt gtggttgcgt gagagcagaa
1081 ggtgacatct ccaatgtggc caatgggttt aagtctcatc tcattcgtct gattggaaat
1141 ctgtgttaca agaataaaga taaccaagac aaggtaaatg agctggatgg tatcccgttg
1201 atcctggaca actgcaacat cagtgacagt aacccctttc tgacccagtg ggtgatatat
1261 gccatccgaa accttaccga agacaacagc caaaaccaag atttgattgc aaagatggag
1321 gaacaggggc tggcagatgc atccctactt aaaaaagtgg gttttgaagt tgaaaagaaa
1381 ggcgaaaagc tgatcctgaa atctactaga gacaccccta agccttaa
//