LOCUS CR457380 1269 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0314D for
gene CRELD1, cysteine-rich with EGF-like domains 1; complete cds,
incl. stopcodon.
ACCESSION CR457380
VERSION CR457380.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1269)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1269)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0314D, ORFNo 2535
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0314D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC008720 we found amino acid
exchange(s) at position (first base of changed triplet):
457(leu->pro) 685(cys->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1269
/db_xref="H-InvDB:HIT000268230"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0314D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1269
/codon_start=1
/gene="CRELD1"
/db_xref="GOA:Q96HD1"
/db_xref="H-InvDB:HIT000268230.13"
/db_xref="HGNC:HGNC:14630"
/db_xref="InterPro:IPR000152"
/db_xref="InterPro:IPR000742"
/db_xref="InterPro:IPR001881"
/db_xref="InterPro:IPR002049"
/db_xref="InterPro:IPR006212"
/db_xref="InterPro:IPR009030"
/db_xref="InterPro:IPR013032"
/db_xref="InterPro:IPR018097"
/db_xref="InterPro:IPR021852"
/db_xref="UniProtKB/Swiss-Prot:Q96HD1"
/protein_id="CAG33661.1"
/translation="MAPWPPKGLVPAVLWGLSLFLNLPGPIWLQPSPPPQSSPPPQPH
PCHTCRGLVDSFNKGLERTIRDNFGGGNTAWEEENLSKYKDSETRLVEVLEGVCSKSD
FECHRLLELSEELVESWWFHKQQEAPDLFQWLCSDSLKLCCPAGTFGPSCPPCPGGTE
RPCGGYGQCEGEGTRGGSGHCDCQAGYGGEACGQCGLGYFEAERNASHLVCSACFGPC
ARCSGPEESNRLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAK
ACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAE
GYKQMEGICVKEQIPGAFPILTDLTPETTRRWKLGSHPHSTYVKMKMQRDEATFPGLY
GKQVAKLGSQSRQSDRGTRLIHSQQASSQR"
BASE COUNT 280 a 346 c 392 g 251 t
ORIGIN
1 atggccccat ggcccccgaa gggcctagtc ccagctgtgc tctggggcct cagcctcttc
61 ctcaacctcc caggacctat ctggctccag ccctctccac ctccccagtc ttctcccccg
121 cctcagcccc atccgtgtca tacctgccgg ggactggttg acagctttaa caagggcctg
181 gagagaacca tccgggacaa ctttggaggt ggaaacactg cctgggagga agagaatttg
241 tccaaataca aagacagtga gacccgcctg gtagaggtgc tggagggtgt gtgcagcaag
301 tcagacttcg agtgccaccg cctgctggag ctgagtgagg agctggtgga gagctggtgg
361 tttcacaagc agcaggaggc cccggacctc ttccagtggc tgtgctcaga ttccctgaag
421 ctctgctgcc ccgcaggcac cttcgggccc tcctgccctc cctgtcctgg gggaacagag
481 aggccctgcg gtggctacgg gcagtgtgaa ggagaaggga cacgaggggg cagcgggcac
541 tgtgactgcc aagccggcta cgggggtgag gcctgtggcc agtgtggcct tggctacttt
601 gaggcagaac gcaacgccag ccatctggta tgttcggctt gttttggccc ctgtgcccga
661 tgctcaggac ctgaggaatc aaaccgtttg caatgcaaga agggctgggc cctgcatcac
721 ctcaagtgtg tagacattga tgagtgtggc acagagggag ccaactgtgg agctgaccaa
781 ttctgcgtga acactgaggg ctcctatgag tgccgagact gtgccaaggc ctgcctaggc
841 tgcatggggg cagggccagg tcgctgtaag aagtgtagcc ctggctatca gcaggtgggc
901 tccaagtgtc tcgatgtgga tgagtgtgag acagaggtgt gtccgggaga gaacaagcag
961 tgtgaaaaca ccgagggcgg ttatcgctgc atctgtgccg agggctacaa gcagatggaa
1021 ggcatctgtg tgaaggagca gatcccaggt gcattcccca tcttaactga tttaacccct
1081 gaaacaaccc gacgctggaa gttgggttct catccccact ctacatacgt aaaaatgaag
1141 atgcagagag atgaagctac tttcccaggg ctatatggca agcaagtcgc aaagctggga
1201 tcccaatcca gacagtctga ccgtggaacg agactcatac acagtcagca ggcttcttct
1261 cagagatga
//