LOCUS CR457365 606 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B1210D for
gene CERK, ceramide kinase; complete cds, incl. stopcodon.
ACCESSION CR457365
VERSION CR457365.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 606)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 606)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B1210D, ORFNo 2498
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B1210D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_182661 we found amino acid
exchange(s) at position (first base of changed triplet):
337(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..606
/db_xref="H-InvDB:HIT000268215"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B1210D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..606
/codon_start=1
/gene="CERK"
/db_xref="H-InvDB:HIT000268215.13"
/db_xref="UniProtKB/TrEMBL:Q6I9Z0"
/protein_id="CAG33646.1"
/translation="MQRGPASGDPREGPPRQRREGCWGLHTCLITAARLRQSGWEHDL
GVCSGSTDDKLWKLAVCKALASLLLLKCQIPMLYIDAKCLTQPGLGAVRRKLAIRGGG
AGPGLPQDSSDAGPEVCTRGPDLSLSFLHTEFSIFIEHLVLLSQSSVNCVSDVCLLPR
SHDGSLVSSAARGLRRPEDSSRKAFLPRSPGHPSIVYYVLV"
BASE COUNT 116 a 165 c 176 g 149 t
ORIGIN
1 atgcaacgtg gccctgcttc aggtgatccg cgggaggggc ctccacgcca gcgccgggaa
61 ggctgctggg gcctccacac ctgcctcatc acggcggcga ggctacgaca atccggctgg
121 gagcatgacc ttggcgtctg ttctgggagc acagatgata agctctggaa gctggcagtg
181 tgtaaagcac tggcaagttt gttactgtta aaatgtcaaa taccaatgct ttatatcgac
241 gcgaagtgct taacacagcc gggcttgggg gcagtcagga ggaagctggc catccgtgga
301 ggaggggccg gtcctggact cccgcaggac tcctctgatg cagggcctga agtctgtaca
361 cgtggtccag atttgtcctt gtcttttctt cacactgagt tctctatatt tattgaacat
421 cttgtccttt taagccagag tagtgtaaac tgcgtctcgg atgtctgtct tttgcctcga
481 agccacgatg gatcgctggt ttcctctgca gcgcgagggc tccggcgacc agaggattct
541 tcccggaagg cattcctgcc gcgctccccg gggcacccct caattgtgta ctacgtcctt
601 gtttaa
//