LOCUS CR457350 777 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0610D for
gene LOC340371, hypothetical protein LOC340371; complete cds, incl.
stopcodon.
ACCESSION CR457350
VERSION CR457350.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 777)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 777)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0610D, ORFNo 2443
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0610D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_178564 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..777
/db_xref="H-InvDB:HIT000268200"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0610D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..777
/codon_start=1
/gene="LOC340371"
/db_xref="GOA:Q9NSY0"
/db_xref="H-InvDB:HIT000268200.12"
/db_xref="HGNC:HGNC:19339"
/db_xref="InterPro:IPR000719"
/db_xref="InterPro:IPR011009"
/db_xref="UniProtKB/Swiss-Prot:Q9NSY0"
/protein_id="CAG33631.1"
/translation="MCALEMAVLEIQTNGDTRVTEEAIARARHSLSDPNMREFILCCL
ARDPARRPSAHSLLFHRVLFEVHSLKLLAAHCFIQHQYLMPENVVEEKTKAMDLHAVL
AELPRPRRPPLQWRYSEVSFMELDKFLEDVRNGIYPLMNFAATRPLGLPRVLAPPPEE
VQKAKTPTPEPFDSETRKVIQMQCNLERSEDKARWHLTLLLVLEDRLHRQLTYDLLPT
DSAQDLASELVHYGFLHEDDRMKLAAFLESTFLKYRGTQA"
BASE COUNT 153 a 262 c 226 g 136 t
ORIGIN
1 atgtgtgcgc tggagatggc tgtactggaa atccagacca atggggacac ccgggtcaca
61 gaggaggcca ttgctcgcgc caggcactcg ctgagtgacc ccaacatgcg ggagttcatc
121 ctttgctgcc tggcccggga ccctgcccgc cggccctctg cccacagcct cctcttccac
181 cgcgtgctct tcgaggtgca ctcgctgaag ctcctggcag cccactgctt catccagcac
241 cagtacctca tgcctgagaa tgtggtggag gagaagacca aggccatgga cctgcacgcg
301 gtcttggcgg agcttccccg gccccgcagg cccccgctgc agtggcggta ctcggaagtc
361 tccttcatgg agctggacaa attcctggag gatgtcagga atggaatcta cccactgatg
421 aactttgcag ccactcgacc cctggggctg ccccgtgtgc tggccccacc cccggaggag
481 gtccaaaagg ccaagacccc gacgccagag ccctttgact ctgagaccag aaaggtcatc
541 cagatgcagt gcaacctgga gagaagcgag gacaaggcgc gctggcatct cactctgctt
601 ctggtgctgg aagaccggct gcaccggcag ctgacctacg acctgctccc aacggacagc
661 gcccaggacc tcgcctcgga gctcgtgcac tatggcttcc tccacgagga cgaccggatg
721 aagctggccg ccttcctgga gagcaccttc ctcaagtacc gtgggaccca ggcttaa
//