LOCUS CR457343 1575 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0521D for
gene FLJ10305, hypothetical protein FLJ10305; complete cds, incl.
stopcodon.
ACCESSION CR457343
VERSION CR457343.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1575)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1575)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0521D, ORFNo 2430
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0521D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AK025479 we found amino acid
exchange(s) at position (first base of changed triplet):
280(thr->ala)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1575
/db_xref="H-InvDB:HIT000268193"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0521D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1575
/codon_start=1
/gene="FLJ10305"
/db_xref="GOA:Q08AM6"
/db_xref="H-InvDB:HIT000268193.12"
/db_xref="HGNC:HGNC:25507"
/db_xref="InterPro:IPR011989"
/db_xref="InterPro:IPR016024"
/db_xref="InterPro:IPR021841"
/db_xref="InterPro:IPR026825"
/db_xref="InterPro:IPR032878"
/db_xref="UniProtKB/Swiss-Prot:Q08AM6"
/protein_id="CAG33624.1"
/translation="MANILVIHCQTTDDLIQLTAMCWMREFIQLAGRVMLPYSSGILT
AVLPCLAYDDRKKSIKEVANVCNQSLMKLVTPEDDELDELRPGQRQAEPAPDDALPKQ
EGTASGGPDGSCDSSFSSGISVFTAASTERAPVTLHLDGIVQVLNCHLSDTAIGMMTR
IAVLKWLYHLYIKTPRKMFRHTDSLFPILLQTLSDESDEVILKDLEVLAEIASSPAGQ
TDDPGPLDGPDLQASHSELQVPTPGRAGLLNTSGTKGLECSPSTPTMNSYFYKFMINL
LKRFSSERKLLEVRGPFIIRQLCLLLNAENIFHSMADILLREEDLKFASTMVHALNTI
LLTSTELFQLRNQLKDLKTLESQNLFCCLYRSWCHNPVTTVSLCFLTQNYRHAYDLIQ
KFGDLEVTVDFLAEVDKLVQLIECPIFTYLRLQLLDVKNNPYLIKALYGLLMLLPQSS
AFQLLSHRLQCVPNPELLQTEDSLKAAPKSQKADSPSIDYAELLQHFEKVQNKHLEVR
HQRSGRGDHLDRRVVL"
BASE COUNT 354 a 512 c 409 g 300 t
ORIGIN
1 atggccaaca tcctggtgat ccactgccag acaacagatg acctcatcca gctgacagcc
61 atgtgctgga tgcgggagtt catccagctg gcgggccgcg tcatgctgcc ttactcctcc
121 gggatcctga ctgctgtctt gccctgcttg gcctacgatg accgcaagaa aagcatcaaa
181 gaagtggcca acgtgtgcaa ccagagcctg atgaagctgg tcacccccga ggacgacgag
241 ctggatgagc tgagacctgg gcagaggcag gcagagcccg cccctgacga tgccctgcca
301 aagcaggagg gcacagccag tggaggtcca gatggttcct gtgactccag cttcagtagc
361 ggcatcagtg tcttcactgc agccagcact gaaagagccc cagtgaccct tcacctcgac
421 gggatcgtgc aggtcctaaa ctgccacctc agtgacacgg ccattgggat gatgaccagg
481 attgcagttc tcaagtggct ctaccacctc tacatcaaaa ctcctcggaa gatgttccgg
541 cacacggaca gcctctttcc catcctactg cagacgttat cggatgaatc ggatgaggtg
601 atcctgaagg acctggaggt gctggcagaa atcgcttcct cccccgcagg ccagacggat
661 gacccaggcc ccctcgatgg ccctgacctc caggccagcc actcagagct ccaggtgccc
721 acccctggca gagccggcct actgaacacc tctggtacca aaggcttaga atgttctcct
781 tcaactccca ccatgaattc ttacttttat aagttcatga tcaaccttct caagagattc
841 agcagcgaac ggaagctcct ggaggtcaga ggccctttca tcatcaggca gctgtgcctc
901 ctgctgaatg cggagaacat cttccactca atggcagaca tcctgctgcg ggaggaggac
961 ctcaagttcg cctcgaccat ggtccacgcc ctcaacacca tcctgctgac ctccacagag
1021 ctcttccagc taaggaacca gctgaaggac ctgaagaccc tggagagcca gaacctgttc
1081 tgctgcctgt accgctcctg gtgccacaac ccagtcacca cggtgtccct ctgcttcctc
1141 acccagaact accggcacgc ctatgacctc atccagaagt ttggggacct ggaggtcacc
1201 gtggacttcc tcgcagaggt ggacaagctg gtgcagctga ttgagtgccc catcttcaca
1261 tatctgcgcc tgcagctgct ggacgtgaag aacaacccct acctgatcaa ggccctctac
1321 ggcctgctca tgctcctgcc gcagagcagc gccttccagc tgctctcgca ccggctccag
1381 tgcgtgccca accctgagct gctgcagacc gaagacagtc taaaggcagc ccccaagtcc
1441 cagaaagctg actcccctag catcgactac gcagagctgc tgcagcactt tgagaaggtc
1501 cagaacaagc acctggaagt gcggcaccag cggagcgggc gtggggacca cctggaccgg
1561 agggttgtcc tttaa
//