LOCUS       CR457336                 867 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834E0613D for
            gene ASB8, ankyrin repeat and SOCS box-containing 8; complete cds,
            incl. stopcodon.
ACCESSION   CR457336
VERSION     CR457336.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 867)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834E0613D, ORFNo 2413
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0613D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC (ATG).
            The last base of the last coding triplett has been changed to T,
            which might lead to an amino acid change at the C terminus of the
            polypeptide.
            The stop codon has been set to TAA followed by
            TTAACCCAGCTTTCTT..att.
            Compared to the reference sequence NM_024095 we found amino acid
            exchange(s) at position (first base of changed triplet):
            862(glu->asp)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..867
                     /db_xref="H-InvDB:HIT000268186"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834E0613D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..867
                     /codon_start=1
                     /gene="ASB8"
                     /db_xref="GOA:Q6IA19"
                     /db_xref="H-InvDB:HIT000268186.13"
                     /db_xref="InterPro:IPR001496"
                     /db_xref="InterPro:IPR002110"
                     /db_xref="InterPro:IPR020683"
                     /db_xref="InterPro:IPR036036"
                     /db_xref="InterPro:IPR036770"
                     /db_xref="InterPro:IPR037332"
                     /db_xref="UniProtKB/TrEMBL:Q6IA19"
                     /protein_id="CAG33617.1"
                     /translation="MSSSMWYIMQSIQSKYSLSERLIRTIAAIRSFPHDNVEDLIRGG
                     ADVNCTHGTLKPLHCACMVSDADCVELLLEKGAEVNALDGYNRTALHYAAEKDEACVE
                     VLLEYGANPNALDGNRDTPLHWAAFKNNAECVRALLESGASVNALDYNNDTPLSWAAM
                     KGNLESVSILLDYGAEVRVINLIGQTPISRLVALLVRGLGTEKEDSCFELLHRAVGHF
                     ELRKNGTMPREVARDPQLCEKLTVLCSAPGTLKTLARYAVRRSLGLQYLPDAVKGLPL
                     PASLKEYLLLLD"
BASE COUNT          219 a          215 c          229 g          204 t
ORIGIN      
        1 atgagttcca gtatgtggta tattatgcag agcattcaga gcaaatactc tctctccgag
       61 cgcttaatcc gaacaattgc tgccatccgt tccttcccac atgataatgt agaggacctc
      121 atcagagggg gagcagatgt gaactgcact catggcacac tgaagccctt gcactgtgcc
      181 tgtatggtgt cagatgctga ctgtgtggag ttacttctgg aaaaaggagc cgaggtgaat
      241 gccctggatg ggtataaccg aacagccctc cactatgcag cagagaaaga tgaggcttgt
      301 gtggaggtcc tattggagta tggtgcaaac cccaatgctt tggatggcaa cagagatacc
      361 ccacttcact gggcagcctt taagaacaat gctgagtgtg tgcgggctct cctagagagc
      421 ggggcctctg tcaatgccct ggattacaac aatgatacac cgctcagctg ggctgccatg
      481 aagggaaatc ttgagagtgt cagcatcctt ctggattatg gcgcagaggt cagagtcatc
      541 aacctaatag gccagacacc catctcccgc ctggtggctc tgctagtcag gggacttgga
      601 acagagaaag aggactcttg ctttgagctc ctccacagag ctgttggaca ctttgaattg
      661 aggaaaaatg gcaccatgcc acgagaggtg gccagagacc cgcagctatg tgaaaaactg
      721 actgttctgt gctcagctcc aggaactcta aaaacactcg ctcgctatgc cgtgcgccgt
      781 agcctgggac tccagtatct ccccgatgca gtgaagggcc ttccactgcc agcttctttg
      841 aaggaatacc tgttactttt agattaa
//