LOCUS CR457326 366 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H109D for
gene SLC35E1, solute carrier family 35, member E1; complete cds,
incl. stopcodon.
ACCESSION CR457326
VERSION CR457326.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 366)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 366)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H109D, ORFNo 2381
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H109D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AK024313 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..366
/db_xref="H-InvDB:HIT000268176"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H109D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..366
/codon_start=1
/gene="SLC35E1"
/db_xref="GOA:Q9H7U6"
/db_xref="H-InvDB:HIT000268176.12"
/db_xref="InterPro:IPR004853"
/db_xref="UniProtKB/TrEMBL:Q9H7U6"
/protein_id="CAG33607.1"
/translation="MPIWVVLLSRIIMKEKQSTKVYLSLIPIISGVLLATVTELSFDM
WGLVSALAATLCFSLQNIFSKKVLRDSRIHHLRLLNILGCHAVFFMIPTWVLVDLSAF
LVSSDLVSCTAPRTRHFLG"
BASE COUNT 68 a 114 c 87 g 97 t
ORIGIN
1 atgcccatct gggtggtcct cctgtcccgg atcattatga aggagaagca gagcaccaag
61 gtatacttgt cactcatccc catcatcagc ggtgtcctgc tggccaccgt caccgagttg
121 tcttttgaca tgtggggact cgtcagcgcc ctcgccgcca cgctgtgctt ctcgcttcag
181 aacattttct ccaaaaaggt cttgcgagat tcacggatcc accatctccg gctgctcaac
241 atcctgggct gccacgccgt cttctttatg atccccacct gggttctggt ggacctctcg
301 gctttcctgg tcagcagcga cttggtgagt tgtacagcac caagaacacg ccattttctt
361 ggttaa
//