LOCUS CR457324 729 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F109D for
gene FLJ14154, hypothetical protein FLJ14154; complete cds, incl.
stopcodon.
ACCESSION CR457324
VERSION CR457324.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 729)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 729)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F109D, ORFNo 2379
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F109D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_024845 we found amino acid
exchange(s) at position (first base of changed triplet):
724(met->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..729
/db_xref="H-InvDB:HIT000268174"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F109D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..729
/codon_start=1
/gene="FLJ14154"
/db_xref="GOA:Q9H7X0"
/db_xref="H-InvDB:HIT000268174.12"
/db_xref="HGNC:HGNC:25875"
/db_xref="InterPro:IPR000182"
/db_xref="InterPro:IPR016181"
/db_xref="PDB:5HGZ"
/db_xref="PDB:5HH0"
/db_xref="PDB:5HH1"
/db_xref="PDB:5ICV"
/db_xref="PDB:5ICW"
/db_xref="UniProtKB/Swiss-Prot:Q9H7X0"
/protein_id="CAG33605.1"
/translation="MTEVVPSSALSEVSLRLLCHDDIDTVKHLCGDWFPIEYPDSWYR
DITSNKKFFSLAATYRGAIVGMIVAEIKNRTKIHKEDGDILASNFSVDTQVAYILSLG
VVKEFRKHGIGSLLLESLKDHISTTAQDHCKAIYLHVLTTNNTAINFYENRDFKQHHY
LPYYYSIRGVLKDGFTYVLYINGGHPPWTILDYIQHLGSALASLSPCSIPHRVYRQAH
SLLCSFLPWSGISSKSGIEYSRTI"
BASE COUNT 193 a 219 c 156 g 161 t
ORIGIN
1 atgacagagg tggtgccatc cagcgcgctc agcgaggtca gcctgcgcct cctctgccac
61 gatgacatag acactgtgaa gcacctgtgt ggcgactggt tccccatcga gtacccagac
121 tcatggtatc gtgatatcac atccaacaag aagttctttt cccttgctgc aacctacaga
181 ggtgccattg tgggaatgat agtagctgaa attaagaaca ggaccaaaat acataaagag
241 gatggagata ttctagcatc caacttctct gttgacacac aagtcgcgta catcctaagt
301 ctgggcgtcg tgaaagagtt caggaagcac ggcataggtt ccctcttact tgaaagttta
361 aaggatcaca tatcaaccac cgcccaggac cactgcaaag ccatttacct gcatgtcctc
421 accaccaaca acacagcaat aaacttctat gaaaacagag acttcaagca gcaccactat
481 ctcccctatt actactccat tcgaggggtc ctcaaagatg gcttcaccta tgtcctctac
541 atcaacggcg ggcaccctcc ctggacgatt ttggactaca tccagcacct gggctctgca
601 ctagccagcc tgagcccctg ctccattccg cacagagtct accgccaggc ccacagcctg
661 ctctgcagct tcctgccatg gtcgggcatc tcttccaaga gtggcatcga gtacagccgg
721 accatttaa
//