LOCUS CR457320 1533 bp mRNA linear HUM 21-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0313D for
gene TOE1, target of EGR1, member 1 (nuclear); complete cds, incl.
stopcodon.
ACCESSION CR457320
VERSION CR457320.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1533)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1533)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F0313D, ORFNo 2371
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0313D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC009364 we found amino acid
exchange(s) at position (first base of changed triplet):
34(ala->thr)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1533
/db_xref="H-InvDB:HIT000268170"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F0313D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1533
/codon_start=1
/gene="TOE1"
/db_xref="GOA:Q96GM8"
/db_xref="H-InvDB:HIT000268170.13"
/db_xref="HGNC:HGNC:15954"
/db_xref="InterPro:IPR000571"
/db_xref="InterPro:IPR006941"
/db_xref="InterPro:IPR012337"
/db_xref="InterPro:IPR036397"
/db_xref="PDB:2FC6"
/db_xref="UniProtKB/Swiss-Prot:Q96GM8"
/protein_id="CAG33601.1"
/translation="MAADSDDGAVSTPAASDGGVSKSTTSGEELVVQVPVVDVQSNNF
KEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGL
ACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHK
GNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADL
CEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSS
MRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAED
KRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNL
PHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYV
MAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWG
SS"
BASE COUNT 380 a 412 c 416 g 325 t
ORIGIN
1 atggccgccg acagtgacga tggcgcagtt tcaactcccg cagcttccga cggtggtgtc
61 agcaaaagca caacatctgg ggaggagcta gtagtccagg ttcccgtagt ggatgtgcaa
121 agcaacaact tcaaggagat gtggccatcc ctcctgctag ccataaagac agctaatttc
181 gtggctgtgg acacggagct gagtgggctt ggggacagga agagtttgct gaaccagtgc
241 attgaggaac gttacaaggc cgtgtgtcat gctgccagga cccgttctat cctttccctg
301 ggcctcgcct gcttcaagcg gcagccagac aagggtgaac attcctatct ggctcaagtg
361 ttcaatctca ctctgctgtg catggaggag tatgtcatag aaccaaagtc tgtgcagttc
421 ctgatacagc atggcttcaa cttcaaccag cagtatgccc aaggcatccc ctaccataag
481 ggcaatgaca agggtgatga gagccagagc cagtcagtac ggaccctatt cctggagcta
541 atccgagccc gccggcccct ggtgctacac aatggcctta tagacttggt gttcctgtac
601 cagaacttct atgcacacct ccctgagagt ctgggaacct tcaccgctga cctgtgtgag
661 atgttcccag caggcattta tgacaccaaa tatgctgctg agtttcatgc ccgtttcgtg
721 gcctcctact tagaatatgc cttccggaaa tgtgaacggg aaaatgggaa gcagcgggca
781 gctggcagcc cacaccttac cctggagttc tgcaactatc cttccagcat gagggaccat
841 attgattacc gctgctgcct gcccccagca acccaccgtc ctcatcccac cagcatctgt
901 gacaacttct cggcttatgg ctggtgcccc ctgggaccac agtgtcctca gtctcacgat
961 attgacctta tcattgacac tgatgaggct gcggcagagg acaagcggcg acggcgacga
1021 cgtagggaaa aacggaagag ggctttattg aacctaccgg ggacacagac ctctggggaa
1081 gctaaggatg gtcctcccaa gaagcaggtc tgtggggata gcatcaagcc tgaagaaacc
1141 gagcaggagg tggctgccga tgaaactagg aacctgcctc actccaagca aggcaacaaa
1201 aatgacttag agatggggat taaggcagca aggcctgaaa tagctgatag agctacctca
1261 gaagtgccag ggagccaagc cagtcctaac ccagtgcctg gggatggatt gcaccgggct
1321 ggttttgatg cctttatgac aggttatgtg atggcctatg tggaagtgag ccagggaccg
1381 cagccctgca gctctggacc ctggctccct gaatgccaca ataaggtata tttgagtggc
1441 aaagctgtac ccctcacagt ggccaagagc cagttctctc gttcctccaa agcccacaat
1501 cagaagatga agctcacttg gggcagtagt taa
//