LOCUS CR457297 1152 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1013D for
gene SAV1, salvador homolog 1 (Drosophila); complete cds, incl.
stopcodon.
ACCESSION CR457297
VERSION CR457297.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1152)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1152)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1013D, ORFNo 2328
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1013D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_021818 we found amino acid
exchange(s) at position (first base of changed triplet):
52(gln->arg)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1152
/db_xref="H-InvDB:HIT000268147"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1013D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1152
/codon_start=1
/gene="SAV1"
/db_xref="GOA:Q9H4B6"
/db_xref="H-InvDB:HIT000268147.13"
/db_xref="HGNC:HGNC:17795"
/db_xref="InterPro:IPR001202"
/db_xref="InterPro:IPR011524"
/db_xref="InterPro:IPR030030"
/db_xref="InterPro:IPR036020"
/db_xref="PDB:6AO5"
/db_xref="UniProtKB/Swiss-Prot:Q9H4B6"
/protein_id="CAG33578.1"
/translation="MLSRKKTKNEVSKPAEVRGKYVKKETSPLLRNLMPSFIRHGPTI
PRRTDICLPDSSPNAFSTSGDVVSRNQSFLRTPIQRTPHEIMRRESNRLSAPSYLARS
LADVPREYGSSQSFVTEVSFAVENGDSGSRYYYSDNFFDGQRKRPLGDRAHEDYRYYE
YNHDLFQRMPQNQGRHASGIGRVAATSLGNLTNHGSEDLPLPPGWSVDWTMRGRKYYI
DHNTNTTHWSHPLEREGLPPGWERVESSEFGTYYVDHTNKKAQYRHPCAPSVPRYDQP
PPVTYQPQQTERNQSLLVPANPYHTAEIPDWLQVYARAPVKYDHILKWELFQLADLDT
YQGMLKLLFMKELEQIVKMYEAYRQALLTELENRKQRQQWYAQQHGKNF"
BASE COUNT 356 a 255 c 249 g 292 t
ORIGIN
1 atgctgtccc gaaagaaaac caaaaacgaa gtgtccaagc cggccgaggt gcgggggaag
61 tacgtgaaga aggagacgtc gcctctgctt cggaatctta tgccttcatt catccggcat
121 ggtccaacaa ttccaagacg aactgatatc tgtcttccag attcaagccc taatgccttt
181 tcaacttctg gagatgtagt ttcaagaaac cagagtttcc ttagaactcc aattcaaaga
241 acacctcatg aaataatgag aagagaaagc aacagattat ctgcaccttc ttatcttgcc
301 agaagtctag cagatgtccc tagagagtat ggttcttctc agtcatttgt aacggaagtt
361 agttttgctg ttgaaaatgg agactctggt tcccgatatt attattcaga caattttttt
421 gatggtcaga gaaagcggcc acttggagat cgtgcacatg aagactacag atattatgaa
481 tacaaccatg atctcttcca aagaatgcca cagaatcagg ggaggcatgc ttcaggtatt
541 gggagagttg ctgctacatc tttaggaaat ttgactaacc atggttctga agatttaccc
601 cttcctcctg gctggtctgt ggactggaca atgagaggga gaaaatatta tatagatcat
661 aacacaaata caactcactg gagccatcct cttgagcgag aaggacttcc tcctggatgg
721 gaacgagttg agtcatccga atttggaacc tattatgtag atcacacaaa taagaaggcc
781 caatacaggc atccctgtgc tcctagtgta cctcggtatg atcaaccacc tcctgtcaca
841 taccagccac agcaaactga aagaaatcag tcccttctgg tacctgcaaa tccatatcat
901 actgcagaaa ttcctgactg gcttcaggtt tacgcacgag cccctgtgaa atatgaccac
961 attctgaagt gggaactctt ccagctggct gacctggata cataccaggg aatgctaaag
1021 ttgctcttca tgaaagaatt ggagcagatt gttaaaatgt atgaagcata cagacaagcc
1081 cttcttacag agttggaaaa ccgaaagcag agacagcagt ggtatgccca acaacatgga
1141 aaaaattttt aa
//