LOCUS CR457290 711 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H049D for
gene FLJ12598, hypothetical protein FLJ12598; complete cds, incl.
stopcodon.
ACCESSION CR457290
VERSION CR457290.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 711)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 711)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H049D, ORFNo 2305
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H049D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_024754 we found amino acid
exchange(s) at position (first base of changed triplet):
706(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..711
/db_xref="H-InvDB:HIT000268140"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H049D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..711
/codon_start=1
/gene="FLJ12598"
/db_xref="GOA:Q8WV60"
/db_xref="H-InvDB:HIT000268140.11"
/db_xref="HGNC:HGNC:25734"
/db_xref="InterPro:IPR002885"
/db_xref="InterPro:IPR011990"
/db_xref="InterPro:IPR034629"
/db_xref="InterPro:IPR034913"
/db_xref="UniProtKB/Swiss-Prot:Q8WV60"
/protein_id="CAG33571.1"
/translation="MKDQHLRGFFSDSTSFNILMDMLFIKGKYKSALQVLIEMKNQDV
KFTKDTYVLAFAICYKLNSPESFKICTTLREEALLKGEILSRRASCFAVALALNQNEM
AKAVSIFSQIMNPESIACINLNIIIHIQSNMLENLIKTLKNAAEGNLSKFVKRHVFSE
EVLAKVREKVKDVPALVAKFDEIYGTLHITGQVTTDSLDAVLCHTPRDRKSHTLLLNK
RMVSRRTFQPLSQSLLAD"
BASE COUNT 227 a 144 c 145 g 195 t
ORIGIN
1 atgaaagacc agcatttacg aggtttcttc tcagactcca catcattcaa tattttgatg
61 gatatgttat ttatcaaagg caaatataaa agtgctttgc aagtattgat agagatgaaa
121 aaccaagatg tgaagttcac caaagatacc tatgttcttg cttttgcaat ttgctacaaa
181 ctgaatagcc ctgagtcttt caaaatctgt actacattaa gagaagaagc tctactcaaa
241 ggagaaattc tctccaggag agcatcctgt ttcgctgtgg cattagctct gaatcagaat
301 gagatggcaa aagctgtgtc cattttttct caaatcatga atccagaaag catagcctgc
361 attaatttaa atattataat ccatatccag tcaaatatgt tggaaaacct gataaagact
421 ctaaaaaatg ctgcagaagg aaatttatca aaatttgtga aaagacatgt gttctcggag
481 gaagtgctgg ccaaagtgag ggaaaaagtg aaggatgtgc ctgcccttgt ggccaaattt
541 gatgagatct atgggacact gcacatcact ggccaggtca ccactgattc tttggatgct
601 gtgctctgcc acacccccag ggacaggaaa tctcacacgt tgctattaaa caagaggatg
661 gtcagccgtc gcaccttcca gccactcagc cagtccctgt tggctgatta a
//