LOCUS       CR457281                 834 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834A0513D for
            gene UCK1, uridine-cytidine kinase 1; complete cds, incl.
            stopcodon.
ACCESSION   CR457281
VERSION     CR457281.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 834)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834A0513D, ORFNo 2276
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0513D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC (ATG).
            The last base of the last coding triplett has been changed to T,
            which might lead to an amino acid change at the C terminus of the
            polypeptide.
            The stop codon has been set to TAA followed by
            TTAACCCAGCTTTCTT..att.
            Compared to the reference sequence AF237290 we found amino acid
            exchange(s) at position (first base of changed triplet):
            523(arg->his)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..834
                     /db_xref="H-InvDB:HIT000268131"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834A0513D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..834
                     /codon_start=1
                     /gene="UCK1"
                     /db_xref="GOA:Q9HA47"
                     /db_xref="H-InvDB:HIT000268131.12"
                     /db_xref="HGNC:HGNC:14859"
                     /db_xref="InterPro:IPR000764"
                     /db_xref="InterPro:IPR006083"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="InterPro:IPR029920"
                     /db_xref="PDB:2JEO"
                     /db_xref="PDB:2UVQ"
                     /db_xref="UniProtKB/Swiss-Prot:Q9HA47"
                     /protein_id="CAG33562.1"
                     /translation="MASAGGEDCESPAPEADRPHQRPFLIGVSGGTASGKSTVCEKIM
                     ELLGQNEVEQRQRKVVILSQDRFYKVLTAEQKAKALKGQYNFDHPDAFDNDLMHRTLK
                     NIVEGKTVEVPTYDFVTHSRLPETTVVYPADVVLFEGILVFYSQEIRDMFHLRLFVDT
                     DSDVRLSRRVLRDVHRGRDLEQILTQYTTFVKPAFEEFCLPTKKYADVIIPRGVDNMV
                     AINLIVQHIQDILNGDICKWHRGGSNGRSYKRTFSEPGDHPGMLTSGKRSHLESSSRP
                     H"
BASE COUNT          195 a          217 c          264 g          158 t
ORIGIN      
        1 atggcttcgg cgggaggcga agactgcgag agccccgcgc cggaggccga ccgtccgcac
       61 cagcggccct tcctgatagg ggtgagcggc ggcactgcca gcgggaagtc gaccgtgtgt
      121 gagaagatca tggagttgct gggacagaac gaggtggaac agcggcagcg gaaggtggtc
      181 atcctgagcc aggacaggtt ctacaaggtc ctgacggcag agcagaaggc caaggccttg
      241 aaaggacagt acaattttga ccatccagat gcctttgata atgatttgat gcacaggact
      301 ctgaagaaca tcgtggaggg caaaacggtg gaggtgccga cctatgattt tgtgacacac
      361 tcaaggttac cagagaccac ggtggtctac cctgcggacg tggttctgtt tgagggcatc
      421 ttggtgttct acagccagga gatccgggac atgttccacc tgcgcctctt cgtggacacc
      481 gactccgacg tcaggctgtc tcgaagagtt ctccgggacg tgcaccgagg gagggacctg
      541 gagcagattc tgacgcagta caccaccttc gtgaagccgg ccttcgagga gttctgcctg
      601 ccgacaaaga agtatgccga tgtgatcatc ccgcgaggag tggacaatat ggttgccatc
      661 aacctgatcg tgcagcacat ccaggacatt ctgaatggtg acatctgcaa atggcaccga
      721 ggagggtcca atgggcggag ctacaagcgg accttttctg agccagggga ccaccctggg
      781 atgctgacct ctggcaaacg gtcacatttg gagtccagca gcagacccca ttaa
//