LOCUS CR457281 834 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0513D for gene UCK1, uridine-cytidine kinase 1; complete cds, incl. stopcodon. ACCESSION CR457281 VERSION CR457281.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 834) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 834) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834A0513D, ORFNo 2276 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0513D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence AF237290 we found amino acid exchange(s) at position (first base of changed triplet): 523(arg->his) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..834 /db_xref="H-InvDB:HIT000268131" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834A0513D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..834 /codon_start=1 /gene="UCK1" /db_xref="GOA:Q9HA47" /db_xref="H-InvDB:HIT000268131.12" /db_xref="HGNC:HGNC:14859" /db_xref="InterPro:IPR000764" /db_xref="InterPro:IPR006083" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR029920" /db_xref="PDB:2JEO" /db_xref="PDB:2UVQ" /db_xref="UniProtKB/Swiss-Prot:Q9HA47" /protein_id="CAG33562.1" /translation="MASAGGEDCESPAPEADRPHQRPFLIGVSGGTASGKSTVCEKIM ELLGQNEVEQRQRKVVILSQDRFYKVLTAEQKAKALKGQYNFDHPDAFDNDLMHRTLK NIVEGKTVEVPTYDFVTHSRLPETTVVYPADVVLFEGILVFYSQEIRDMFHLRLFVDT DSDVRLSRRVLRDVHRGRDLEQILTQYTTFVKPAFEEFCLPTKKYADVIIPRGVDNMV AINLIVQHIQDILNGDICKWHRGGSNGRSYKRTFSEPGDHPGMLTSGKRSHLESSSRP H" BASE COUNT 195 a 217 c 264 g 158 t ORIGIN 1 atggcttcgg cgggaggcga agactgcgag agccccgcgc cggaggccga ccgtccgcac 61 cagcggccct tcctgatagg ggtgagcggc ggcactgcca gcgggaagtc gaccgtgtgt 121 gagaagatca tggagttgct gggacagaac gaggtggaac agcggcagcg gaaggtggtc 181 atcctgagcc aggacaggtt ctacaaggtc ctgacggcag agcagaaggc caaggccttg 241 aaaggacagt acaattttga ccatccagat gcctttgata atgatttgat gcacaggact 301 ctgaagaaca tcgtggaggg caaaacggtg gaggtgccga cctatgattt tgtgacacac 361 tcaaggttac cagagaccac ggtggtctac cctgcggacg tggttctgtt tgagggcatc 421 ttggtgttct acagccagga gatccgggac atgttccacc tgcgcctctt cgtggacacc 481 gactccgacg tcaggctgtc tcgaagagtt ctccgggacg tgcaccgagg gagggacctg 541 gagcagattc tgacgcagta caccaccttc gtgaagccgg ccttcgagga gttctgcctg 601 ccgacaaaga agtatgccga tgtgatcatc ccgcgaggag tggacaatat ggttgccatc 661 aacctgatcg tgcagcacat ccaggacatt ctgaatggtg acatctgcaa atggcaccga 721 ggagggtcca atgggcggag ctacaagcgg accttttctg agccagggga ccaccctggg 781 atgctgacct ctggcaaacg gtcacatttg gagtccagca gcagacccca ttaa //